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Applied and Environmental Microbiology, August 2006, p. 5376-5383, Vol. 72, No. 8
0099-2240/06/$08.00+0     doi:10.1128/AEM.00091-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Intraspecific Genotypic Characterization of Lactobacillus rhamnosus Strains Intended for Probiotic Use and Isolates of Human Origin

M. Vancanneyt,1* G. Huys,2 K. Lefebvre,1 V. Vankerckhoven,3 H. Goossens,3,4 and J. Swings1,2

BCCM/LMG Bacteria Collection,1 Laboratory of Microbiology, Ghent University, Ghent, Belgium,2 Laboratory of Medical Microbiology, University of Antwerp, Antwerp, Belgium,3 Leiden University Medical Center, Leiden, The Netherlands4

Received 13 January 2006/ Accepted 11 May 2006

A set of 118 strains of the species Lactobacillus rhamnosus was collected, including probiotic strains, research strains with potential probiotic properties, food starter cultures, and human isolates. The majority of the strains were collected from companies, hospitals, or culture collections or were obtained after contacting authors who reported clinical case studies in the literature. The present work aimed to reveal the genotypic relationships between strains of these diverse sources. All strains were initially investigated using fluorescent amplified fragment length polymorphism (FAFLP) with three different primer combinations. Numerical analysis of FAFLP data allowed (i) confirmation of the identification of all strains as members of L. rhamnosus and (ii) delineation of seven stable intraspecific FAFLP clusters. Most of these clusters contained both (potentially) probiotic strains and isolates of human origin. For each of the clusters, strains of different sources were selected for pulsed-field gel electrophoresis (PFGE) of macrorestriction fragments obtained with the enzymes NotI and AscI. Analysis of PFGE data indicated that (i) some (potentially) probiotic strains were indistinguishable from other probiotic strains, suggesting that several companies may use duplicate cultures of the same probiotic strain, and (ii) in a number of cases human isolates from sterile body sites were indistinguishable from a particular probiotic strain, suggesting that some of these isolates may be reisolations of commercial strains.

* Corresponding author. Mailing address: BCCM/LMG Bacteria Collection, Ghent University, Laboratory of Microbiology, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium. Phone: 32 9 2645115. Fax: 32 9 2645092. E-mail: Marc.Vancanneyt{at}UGent.be.

Applied and Environmental Microbiology, August 2006, p. 5376-5383, Vol. 72, No. 8
0099-2240/06/$08.00+0     doi:10.1128/AEM.00091-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Vankerckhoven, V., Huys, G., Vancanneyt, M., Snauwaert, C., Swings, J., Klare, I., Witte, W., Van Autgaerden, T., Chapelle, S., Lammens, C., Goossens, H. (2008). Genotypic Diversity, Antimicrobial Resistance, and Virulence Factors of Human Isolates and Probiotic Cultures Constituting Two Intraspecific Groups of Enterococcus faecium Isolates. Appl. Environ. Microbiol. 74: 4247-4255 [Abstract] [Full Text]  
  • Vankerckhoven, V., Moreillon, P., Piu, S., Giddey, M., Huys, G., Vancanneyt, M., Goossens, H., Entenza, J. M. (2007). Infectivity of Lactobacillus rhamnosus and Lactobacillus paracasei isolates in a rat model of experimental endocarditis. J Med Microbiol 56: 1017-1024 [Abstract] [Full Text]  


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