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Applied and Environmental Microbiology, August 2006, p. 5500-5511, Vol. 72, No. 8
0099-2240/06/$08.00+0     doi:10.1128/AEM.00237-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Identification and Preliminary Characterization of Two cDNAs Encoding Unique Carbonic Anhydrases from the Marine Alga Emiliania huxleyi

Amelia R. Soto,{dagger} Hong Zheng, Dorinda Shoemaker, Jason Rodriguez, Betsy A. Read, and Thomas M. Wahlund*

Department of Biological Sciences, California State University—San Marcos, 333 S. Twin Oaks Valley Road, San Marcos, California 92096-0001

Received 30 January 2006/ Accepted 25 May 2006

Marine coccolithophorid algae are thought to play a significant role in carbon cycling due to their ability to incorporate dissolved inorganic carbon (DIC) into both calcite and photosynthetic products. Among coccolithophorids, Emiliania huxleyi is the most prolific, forming massive blooms that affect the global environment. In addition to its ecological importance, the elaborate calcite structures (coccoliths) are being investigated for the design of potential materials for science and biotechnological devices. To date, most of the research focus in this organism has involved the partitioning of DIC between calcification and photosynthesis, primarily using measurements of an external versus internal carbonic anhydrase (CA) activity under defined conditions. The actual genes, proteins, and pathways employed in these processes have not been identified and characterized (see the work of Quinn et al. in this issue [P. Quinn, R. M. Bowers, X. Zhang, T. M. Wahlund, M. A. Fanelli, D. Olszova, and B. A. Read, Appl. Environ. Microbiol. 72:5512-5526, 2006]). In this study, the cloning and preliminary characterization of two genetically distinct carbonic anhydrase cDNAs are described. Phylogenetic analysis indicated that these two genes belonged to the gamma ({gamma}-EhCA2) and delta ({delta}-EhCA1) classes of carbonic anhydrases. The deduced amino acid sequence of {delta}-EhCA1 revealed that it encodes a protein of 702 amino acids (aa) (ca. 77.3 kDa), with a transmembrane N-terminal region of 373 aa and an in-frame C-terminal open reading frame of 329 aa that defines the CA region. The {gamma}-EhCA2 protein was 235 aa in length (ca. 24.9 kDa) and was successfully expressed in Escherichia coli BL21(DE3) and purified as an active recombinant CA. The expression levels of each transcript from quantitative reverse transcription-PCR experiments under bicarbonate limitation and over a 24-h time course suggest that these isozymes perform different functions in E. huxleyi.

* Corresponding author. Mailing address: Department of Biological Sciences, California State University—San Marcos, 333 S. Twin Oaks Valley Road, San Marcos, CA 92096-0001. Phone: (760) 750-8042. Fax: (760) 750-3063. E-mail: twahlund{at}csusm.edu.

{dagger} Present address: Department of Biochemistry, University of California at Irvine, Irvine, Calif.

Applied and Environmental Microbiology, August 2006, p. 5500-5511, Vol. 72, No. 8
0099-2240/06/$08.00+0     doi:10.1128/AEM.00237-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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