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Applied and Environmental Microbiology, August 2006, p. 5626-5630, Vol. 72, No. 8
0099-2240/06/$08.00+0     doi:10.1128/AEM.00586-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Development of a Dinoflagellate-Oriented PCR Primer Set Leads to Detection of Picoplanktonic Dinoflagellates from Long Island Sound{dagger}

Senjie Lin,1* Huan Zhang,1 Yubo Hou,1 Lilibeth Miranda,1 and Debashish Bhattacharya2

Department of Marine Sciences, University of Connecticut, Groton, Connecticut 06340,1 Department of Biological Sciences and Roy J. Carver Center for Comparative Genomics, University of Iowa, 446 Biology Building, Iowa City, Iowa 52242-13242

Received 12 March 2006/ Accepted 18 May 2006

We developed dinoflagellate-specific 18S rRNA gene primers. PCR amplification using these oligonucleotides for a picoplanktonic DNA sample from Long Island Sound yielded 24 clones, and all but one of these clones were dinoflagellates primarily belonging to undescribed and Amoebophrya-like lineages. These results highlight the need for a systematic investigation of picodinoflagellate diversity in both coastal and oceanic ecosystems.

* Corresponding author. Mailing address: Department of Marine Sciences, University of Connecticut, Groton, CT 06340. Phone: (860) 405-9168. Fax: (860) 405-9153. E-mail: senjie.lin{at}uconn.edu.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, August 2006, p. 5626-5630, Vol. 72, No. 8
0099-2240/06/$08.00+0     doi:10.1128/AEM.00586-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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