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Applied and Environmental Microbiology, August 2006, p. 5659-5661, Vol. 72, No. 8
0099-2240/06/$08.00+0     doi:10.1128/AEM.01057-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Molecular Biological Detection of Anaerobic Gut Fungi (Neocallimastigales) from Landfill Sites

Robert J. Lockhart,1 Michele I. Van Dyke,1,{dagger} Ian R. Beadle,2,{ddagger} Paul Humphreys,2,§ and Alan J. McCarthy1*

School of Biological Sciences, Biosciences Building, University of Liverpool, Liverpool L69 7ZB, United Kingdom,1 Environmental Assessments, BNFL, Risley, Warrington WA3 6AS, United Kingdom2

Received 8 May 2006/ Accepted 9 June 2006

Oligonucleotide primers were designed for the 18S rRNA genes of members of the Neocallimastigales and used in a nested PCR protocol to amplify 787-bp fragments of DNA from landfill site samples. The specificities of the primers were confirmed by phylogenetic analysis of the environmental clone sequences, and this method can therefore now be used to investigate the ecology of the obligately anaerobic fungi. To our knowledge, this is the first demonstration of the occurrence of members of the Neocallimastigales outside the mammalian gut, and their distribution across the landfill samples examined here suggests that they are actively involved in cellulose degradation.

* Corresponding author. Mailing address: School of Biological Sciences, Biosciences Building, University of Liverpool, Liverpool L69 7ZB, United Kingdom. Phone: (44) 151 795 4574. Fax: (44) 151 795 4410. E-mail: aj55m{at}liv.ac.uk.

{dagger} Present address: Department of Civil Engineering, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1.

{ddagger} Present address: AMEC Earth and Environmental Services, Sankey House, 130, Birchwood Blvd., Warrington WA3 7QH, United Kingdom.

§ Present address: School of Geography and Earth Sciences, University of Huddersfield, Queensgate, Huddersfield HD1 3DH, United Kingdom.

Applied and Environmental Microbiology, August 2006, p. 5659-5661, Vol. 72, No. 8
0099-2240/06/$08.00+0     doi:10.1128/AEM.01057-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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