This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lockhart, R. J.
Right arrow Articles by McCarthy, A. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lockhart, R. J.
Right arrow Articles by McCarthy, A. J.
Agricola
Right arrow Articles by Lockhart, R. J.
Right arrow Articles by McCarthy, A. J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 2006, p. 5659-5661, Vol. 72, No. 8
0099-2240/06/$08.00+0     doi:10.1128/AEM.01057-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Molecular Biological Detection of Anaerobic Gut Fungi (Neocallimastigales) from Landfill Sites

Robert J. Lockhart,1 Michele I. Van Dyke,1,{dagger} Ian R. Beadle,2,{ddagger} Paul Humphreys,2,§ and Alan J. McCarthy1*

School of Biological Sciences, Biosciences Building, University of Liverpool, Liverpool L69 7ZB, United Kingdom,1 Environmental Assessments, BNFL, Risley, Warrington WA3 6AS, United Kingdom2

Received 8 May 2006/ Accepted 9 June 2006

Oligonucleotide primers were designed for the 18S rRNA genes of members of the Neocallimastigales and used in a nested PCR protocol to amplify 787-bp fragments of DNA from landfill site samples. The specificities of the primers were confirmed by phylogenetic analysis of the environmental clone sequences, and this method can therefore now be used to investigate the ecology of the obligately anaerobic fungi. To our knowledge, this is the first demonstration of the occurrence of members of the Neocallimastigales outside the mammalian gut, and their distribution across the landfill samples examined here suggests that they are actively involved in cellulose degradation.

* Corresponding author. Mailing address: School of Biological Sciences, Biosciences Building, University of Liverpool, Liverpool L69 7ZB, United Kingdom. Phone: (44) 151 795 4574. Fax: (44) 151 795 4410. E-mail: aj55m{at}liv.ac.uk.

{dagger} Present address: Department of Civil Engineering, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1.

{ddagger} Present address: AMEC Earth and Environmental Services, Sankey House, 130, Birchwood Blvd., Warrington WA3 7QH, United Kingdom.

§ Present address: School of Geography and Earth Sciences, University of Huddersfield, Queensgate, Huddersfield HD1 3DH, United Kingdom.

Applied and Environmental Microbiology, August 2006, p. 5659-5661, Vol. 72, No. 8
0099-2240/06/$08.00+0     doi:10.1128/AEM.01057-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • McDonald, J. E., de Menezes, A. B., Allison, H. E., McCarthy, A. J. (2009). Molecular Biological Detection and Quantification of Novel Fibrobacter Populations in Freshwater Lakes. Appl. Environ. Microbiol. 75: 5148-5152 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»