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Applied and Environmental Microbiology, August 2006, p. 5673-5676, Vol. 72, No. 8
0099-2240/06/$08.00+0     doi:10.1128/AEM.01894-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Cloning and Expression of Two Crystal Protein Genes, cry30Ba1 and cry44Aa1, Obtained from a Highly Mosquitocidal Strain, Bacillus thuringiensis subsp. entomocidus INA288

Takeshi Ito, Tomonori Ikeya, Ken Sahara, Hisanori Bando, and Shin-ichiro Asano

Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan

Received 10 August 2005/ Accepted 12 June 2006

Two novel crystal protein genes, cry30Ba and cry44Aa, were cloned from Bacillus thuringiensis subsp. entomocidus INA288 and expressed in an acrystalliferous strain. Cry44Aa crystals were highly toxic to second-instar Culex pipiens pallens (50% mortality concentration [LC50] = 6 ng/ml) and Aedes aegypti (LC50 = 12 ng/ml); however, Cry30Ba crystals were not toxic.

* Corresponding author. Mailing address: Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. Phone and fax: 81-011-706-2487. E-mail: ito-t{at}abs.agr.hokudai.ac.jp.

Applied and Environmental Microbiology, August 2006, p. 5673-5676, Vol. 72, No. 8
0099-2240/06/$08.00+0     doi:10.1128/AEM.01894-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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