This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Moss, J. A.
Right arrow Articles by Snyder, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Moss, J. A.
Right arrow Articles by Snyder, R. A.
Agricola
Right arrow Articles by Moss, J. A.
Right arrow Articles by Snyder, R. A.

Next Article 

Applied and Environmental Microbiology, September 2006, p. 5679-5688, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.02773-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Stability and Change in Estuarine Biofilm Bacterial Community Diversity

Joseph A. Moss, Andreas Nocker, Joe E. Lepo, and Richard A. Snyder*

Center for Environmental Diagnostics and Bioremediation, University of West Florida, Pensacola, Florida 32514

Received 23 November 2005/ Accepted 15 May 2006

Biofouling communities contribute significantly to aquatic ecosystem productivity and biogeochemical cycling. Our knowledge of the distribution, composition, and activities of these microbially dominated communities is limited compared to other components of estuarine ecosystems. This study investigated the temporal stability and change of the dominant phylogenetic groups of the domain Bacteria in estuarine biofilm communities. Glass slides were deployed monthly over 1 year for 7-day incubations during peak tidal periods in East Sabine Bay, Fla. Community profiling was achieved by using 16S rRNA genes and terminal restriction fragment length polymorphism (T-RFLP) of 16S rRNA genes in combination with ribotyping, cloning, and sequencing to evaluate diversity and to identify dominant microorganisms. Bacterial community profiles from biofilms grown near the benthos showed distinct periods of constancy within winter and summer sampling periods. Similar periods of stability were also seen in T-RFLP patterns from floating biofilms. Alternating dominance of phylogenetic groups between seasons appeared to be associated with seasonal changes in temperature, nutrient availability, and light. The community structure appeared to be stable during these periods despite changes in salinity and in dissolved oxygen.

* Corresponding author. Mailing address: Center for Environmental Diagnostics and Bioremediation, University of West Florida, Pensacola, FL 32514. Phone: (850) 474-2060. Fax: (850) 474-3130. E-mail: rsnyder{at}uwf.edu.

Applied and Environmental Microbiology, September 2006, p. 5679-5688, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.02773-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Collins, R. E., Rocap, G. (2007). REPK: an analytical web server to select restriction endonucleases for terminal restriction fragment length polymorphism analysis. Nucleic Acids Res 35: W58-W62 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»