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Applied and Environmental Microbiology, September 2006, p. 5728-5733, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.00707-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Diversity of Telomere Palindromic Sequences and Replication Genes among Streptomyces Linear Plasmids

Ran Zhang,1 Yong Yang,1 Ping Fang,2 Chenglin Jiang,3 Lihua Xu,3 Yingmin Zhu,1 Meijuan Shen,1 Haiyang Xia,1 Jianfu Zhao,2 Tongbin Chen,4 and Zhongjun Qin1*

Shanghai Institute of Plant Physiology, Shanghai Institutes of Biological Science, Chinese Academy of Sciences, Shanghai,1 College of Environmental Sciences & Engineering, National Key Laboratory of Pollution Control & Resources Reuse, Tongji University, Shanghai,2 Institute of Microbiology, Yunnan University, Kunming,3 Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, People's Republic of China4

Received 28 March 2006/ Accepted 8 June 2006

Streptomyces sp. linear plasmids and linear chromosomes usually contain conserved terminal palindromic sequences bound by the conserved telomeric proteins Tap and Tp, encoded by the tap and tpg genes, respectively, as well as plasmid loci required for DNA replication in circular mode when the telomeres are deleted. These consist of iterons and an adjacent rep gene. By using PCR, we found that 8 of 17 newly detected linear plasmids in Streptomyces strains lack typical telomeric tap and tpg sequences. Instead, two novel telomeres in plasmids pRL1 and pRL2 from the eight strains and one conserved telomere in pFRL1 from the other strains were identified, while multiple short palindromes were also found in the plasmids. The complete nucleotide sequence of pRL2 revealed a gene encoding a protein containing two domains, resembling Tap of Streptomyces and a helicase of Thiobacillus, and an adjacent gene encoding a protein similar to Tpg of Streptomyces and a portion of the telomere terminal protein pTP of adenoviruses. No typical iterons-rep loci were found in the three plasmids. These results indicate an unexpected diversity of telomere palindromic sequences and replication genes among Streptomyces linear plasmids.

* Corresponding author. Mailing address: Shanghai Institute of Plant Physiology, Shanghai Institutes of Biological Science, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, People's Republic of China. Phone: 86-21-54924171. Fax: 86-21-54924171.E-mail: qin{at}sibs.ac.cn.

Applied and Environmental Microbiology, September 2006, p. 5728-5733, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.00707-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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