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Applied and Environmental Microbiology, September 2006, p. 5998-6003, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.00979-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Effects of Chloromethanes on Growth of and Deletion of the pce Gene Cluster in Dehalorespiring Desulfitobacterium hafniense Strain Y51

Taiki Futagami, Takehito Yamaguchi, Shun-ichi Nakayama, Masatoshi Goto, and Kensuke Furukawa*

Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan

Received 26 April 2006/ Accepted 23 June 2006

The dehalorespiring Desulfitobacterium hafniense strain Y51 efficiently dechlorinates tetrachloroethene (PCE) to cis-1,2-dichloroethene (cis-DCE) via trichloroethene by PceA reductive dehalogenase encoded by the pceA gene. In a previous study, we found that the significant growth inhibition of strain Y51 occurred in the presence of commercial cis-DCE. In this study, it turned out that the growth inhibition was caused by chloroform (CF) contamination of cis-DCE. Interestingly, CF did not affect the growth of PCE-nondechlorinating SD (small deletion) and LD (large deletion) variants, where the former fails to transcribe the pceABC genes caused by a deletion of the promoter and the latter lost the entire pceABCT gene cluster. Therefore, PCE-nondechlorinating variants, mostly LD variant, became predominant, and dechlorination activity was significantly reduced in the presence of CF. Moreover, such a growth inhibitory effect was also observed in the presence of carbon tetrachloride at 1 µM, but not carbon dichloride even at 1 mM.

* Corresponding author. Mailing address: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan. Phone and fax: 81-92-642-2849. E-mail: kfurukaw{at}agr.kyushu-u.ac.jp.

Applied and Environmental Microbiology, September 2006, p. 5998-6003, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.00979-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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Copyright © 2006 by the American Society for Microbiology. All rights reserved.