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Applied and Environmental Microbiology, September 2006, p. 6034-6039, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.00897-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Novel Intermediates of Acenaphthylene Degradation by Rhizobium sp. Strain CU-A1: Evidence for Naphthalene-1,8-Dicarboxylic Acid Metabolism

Siriwat Poonthrigpun,1 Kobchai Pattaragulwanit,1,2* Sarunya Paengthai,1 Thanyanuch Kriangkripipat,1 Kanchana Juntongjin,1,2 Suthep Thaniyavarn,1,2 Amorn Petsom,3 and Pairoh Pinphanichakarn1,2

Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand,1 National Research Center for Environmental and Hazardous Waste Management (NRC-EHWM), Faculty of Science, Chulalongkorn University, Bangkok, Thailand,2 Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand3

Received 17 April 2006/ Accepted 18 July 2006

The acenaphthylene-degrading bacterium Rhizobium sp. strain CU-A1 was isolated from petroleum-contaminated soil in Thailand. This strain was able to degrade 600 mg/liter acenaphthylene completely within three days. To elucidate the pathway for degradation of acenaphthylene, strain CU-A1 was mutagenized by transposon Tn5 in order to obtain mutant strains deficient in acenaphthylene degradation. Metabolites produced from Tn5-induced mutant strains B1, B5, and A53 were purified by thin-layer chromatography and silica gel column chromatography and characterized by mass spectrometry. The results suggested that this strain cleaved the fused five-membered ring of acenaphthylene to form naphthalene-1,8-dicarboxylic acid via acenaphthenequinone. One carboxyl group of naphthalene-1,8-dicarboxylic acid was removed to form 1-naphthoic acid which was transformed into salicylic acid before metabolization to gentisic acid. This work is the first report of complete acenaphthylene degradation by a bacterial strain.

* Corresponding author. Mailing address: Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. Phone: 66-2218-5070. Fax: 66-2252-7576. E-mail: Kobchai1a{at}yahoo.com.

Applied and Environmental Microbiology, September 2006, p. 6034-6039, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.00897-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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