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Applied and Environmental Microbiology, September 2006, p. 6325-6330, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.03056-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Detection of Enterobacter sakazakii in Dried Infant Milk Formula by Cationic-Magnetic-Bead Capture

N. R. Mullane,1 J. Murray,2 D. Drudy,1 N. Prentice,2 P. Whyte,1 P. G. Wall,3 A. Parton,2 and S. Fanning1*

Centre for Food Safety, School of Agriculture, Food Science and Veterinary Medicine,1 School of Public Health & Population Science, University College Dublin, Belfield, Dublin 4, Ireland,3 Matrix Microscience Ltd., Lynx Business Park, Fordham Road, Newmarket, Cambridgeshire CB8 7NY, United Kingdom2

Received 28 December 2005/ Accepted 28 June 2006

Enterobacter sakazakii has been associated with life-threatening infections in premature low-birth-weight infants. Contaminated infant milk formula (IMF) has been implicated in cases of E. sakazakii meningitis. Quick and sensitive methods to detect low-level contamination sporadically present in IMF preparations would positively contribute towards risk reduction across the infant formula food chain. Here we report on the development of a simple method, combining charged separation and growth on selective agar, to detect E. sakazakii in IMF. This protocol can reliably detect 1 to 5 CFU of E. sakazakii in 500 g of IMF in less than 24 h.

* Corresponding author. Mailing address: Centre for Food Safety, School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland. Phone: 353-1 716 6082. Fax: 353-1 716 6091. E-mail: sfanning{at}ucd.ie.

Applied and Environmental Microbiology, September 2006, p. 6325-6330, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.03056-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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