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Applied and Environmental Microbiology, September 2006, p. 6371-6376, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.00701-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Molecular Diversity, Cultivation, and Improved Detection by Fluorescent In Situ Hybridization of a Dominant Group of Human Gut Bacteria Related to Roseburia spp. or Eubacterium rectale

Rustam I. Aminov,1 Alan W. Walker,1 Sylvia H. Duncan,1 Hermie J. M. Harmsen,2 Gjalt W. Welling,2 and Harry J. Flint1*

Gut Health Division, Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, United Kingdom,1 Department of Medical Microbiology, University of Groningen, 9700 RB Groningen, The Netherlands2

Received 27 March 2006/ Accepted 26 June 2006

Phylogenetic analysis was used to compare 16S rRNA sequences from 19 cultured human gut strains of Roseburia and Eubacterium rectale with 356 related sequences derived from clone libraries. The cultured strains were found to represent five of the six phylotypes identified. A new oligonucleotide probe, Rrec584, and the previous group probe Rint623, when used in conjunction with a new helper oligonucleotide, each recognized an average of 7% of bacteria detected by the eubacterial probe Eub338 in feces from 10 healthy volunteers. Most of the diversity within this important group of butyrate-producing gut bacteria can apparently be retrieved through cultivation.

* Corresponding author. Mailing address: Gut Health Division, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, United Kingdom. Phone: 44 (1224) 716643. Fax: 44 (1224) 716687. E-mail: hjf{at}rri.sari.ac.uk.

Applied and Environmental Microbiology, September 2006, p. 6371-6376, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.00701-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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