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Applied and Environmental Microbiology, September 2006, p. 6388-6393, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.02218-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Complementation of a Clostridium perfringens spo0A Mutant with Wild-Type spo0A from Other Clostridium Species

I-Hsiu Huang and Mahfuzur R. Sarker*

Department of Biomedical Sciences, College of Veterinary Medicine, and Department of Microbiology, College of Science, Oregon State University, Corvallis, Oregon 97331

Received 19 September 2005/ Accepted 16 June 2006

To evaluate whether C. perfringens can be used as a model organism for studying the sporulation process in other clostridia, C. perfringens spo0A mutant IH101 was complemented with wild-type spo0A from four different Clostridium species. Wild-type spo0A from C. acetobutylicum or C. tetani, but not from C. botulinum or C. difficile, restored sporulation and enterotoxin production in IH101. The ability of spo0A from C. botulinum or C. difficile to complement the lack of spore formation in IH101 might be due, at least in part, to the low levels of spo0A transcription and Spo0A production.

* Corresponding author. Mailing address: Department of Biomedical Sciences, Oregon State University, 216 Dryden Hall, Corvallis, OR 97331. Phone: (541) 737-6918. Fax: (541) 737-2730. E-mail: sarkerm{at}oregonstate.edu.

Applied and Environmental Microbiology, September 2006, p. 6388-6393, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.02218-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Harry, K. H., Zhou, R., Kroos, L., Melville, S. B. (2009). Sporulation and Enterotoxin (CPE) Synthesis Are Controlled by the Sporulation-Specific Sigma Factors SigE and SigK in Clostridium perfringens. J. Bacteriol. 191: 2728-2742 [Abstract] [Full Text]  
  • Paredes-Sabja, D., Torres, J. A., Setlow, P., Sarker, M. R. (2008). Clostridium perfringens Spore Germination: Characterization of Germinants and Their Receptors. J. Bacteriol. 190: 1190-1201 [Abstract] [Full Text]  


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