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Applied and Environmental Microbiology, September 2006, p. 6414-6416, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.01084-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Seleno-L-Methionine Is the Predominant Organic Form of Selenium in Cupriavidus metallidurans CH34 Exposed to Selenite or Selenate{dagger}

Laure Avoscan,1 Richard Collins,1,{ddagger} Marie Carriere,1 Barbara Gouget,1* and Jacques Covès2*

Laboratoire Pierre Süe, CEA/CNRS UMR 9956, 91191 Gif sur Yvette,1 Laboratoire des Protéines Membranaires, Institut de Biologie Structurale—Jean-Pierre Ebel, UMR 5075 CNRS-CEA-UJF, 41, rue Jules Horowitz, 38027 Grenoble Cedex, France2

Received 5 May 2006/ Accepted 21 June 2006

The accumulated organic form of selenium previously detected by X-ray absorption near-edge structure (XANES) analyses in Cupriavidus metallidurans CH34 exposed to selenite or selenate was identified as seleno-L-methionine by coupling high-performance liquid chromatography to inductively coupled plasma-mass spectrometry.


* Corresponding author. Mailing address for J. Covès: Laboratoire des Protéines Membranaires, Institut de Biologie Structurale—Jean-Pierre Ebel, UMR 5075 CNRS-CEA-UJF, 41, rue Jules Horowitz, 38027 Grenoble Cedex, France. Phone: 33 (0) 4-38-78-24-03. Fax: 33 (0) 4-38-78-54-94. E-mail: jacques.coves{at}ibs.fr. Mailing address for B. Gouget: Laboratoire Pierre Süe, CEA/CNRS UMR 9956, 91191 Gif sur Yvette, France. Phone: 33 (0) 1-69-08-33-13. Fax: 33 (0) 1-69-08-69-23. E-mail: barbara.gouget{at}cea.fr.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} Present address: Centre for Water and Waste Technology, School of Civil and Environmental Engineering, The University of New South Wales, Sydney 2052, Australia.


Applied and Environmental Microbiology, September 2006, p. 6414-6416, Vol. 72, No. 9
0099-2240/06/$08.00+0     doi:10.1128/AEM.01084-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.




This article has been cited by other articles:

  • Avoscan, L., Carriere, M., Proux, O., Sarret, G., Degrouard, J., Coves, J., Gouget, B. (2009). Enhanced Selenate Accumulation in Cupriavidus metallidurans CH34 Does Not Trigger a Detoxification Pathway. Appl. Environ. Microbiol. 75: 2250-2252 [Abstract] [Full Text]