AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
AEM.02985-06v1
73/10/3205    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Podar, M.
Right arrow Articles by Keller, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Podar, M.
Right arrow Articles by Keller, M.
Agricola
Right arrow Articles by Podar, M.
Right arrow Articles by Keller, M.
Applied and Environmental Microbiology, May 2007, p. 3205-3214, Vol. 73, No. 10
0099-2240/07/$08.00+0     doi:10.1128/AEM.02985-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Targeted Access to the Genomes of Low-Abundance Organisms in Complex Microbial Communities{triangledown}

Mircea Podar,1,2 Carl B. Abulencia,1 Marion Walcher,1 Don Hutchison,1 Karsten Zengler,1 Joseph A. Garcia,1 Trevin Holland,1 David Cotton,1 Loren Hauser,2 and Martin Keller1,2*

Diversa Corporation, 4955 Directors Place, San Diego, California 92121,1 Oak Ridge National Laboratory, Biosciences Division, Oak Ridge, Tennessee 378312

Received 22 December 2006/ Accepted 12 March 2007

Current metagenomic approaches to the study of complex microbial consortia provide a glimpse into the community metabolism and occasionally allow genomic assemblies for the most abundant organisms. However, little information is gained for the members of the community present at low frequencies, especially those representing yet-uncultured taxa, which include the bulk of the diversity present in most environments. Here we used phylogenetically directed cell separation by fluorescence in situ hybridization and flow cytometry, followed by amplification and sequencing of a fraction of the genomic DNA of several bacterial cells that belong to the TM7 phylum. Partial genomic assembly allowed, for the first time, a look into the evolution and potential metabolism of a soil representative from this group of organisms for which there are no species in stable laboratory cultures. Genomic reconstruction from targeted cells of uncultured organisms isolated directly from the environment represents a powerful approach to access any specific members of a community and an alternative way to assess the community's metabolic potential.

* Corresponding author. Mailing address: Oak Ridge National Laboratory, Biosciences Division, One Bethel Valley Road, P.O. Box 2008, MS-6035, Oak Ridge, TN 37831-6026. Phone: (865) 576-0567. Fax: (865) 576-0524. E-mail: kellerm{at}ornl.gov

{triangledown} Published ahead of print on 16 March 2007.

Applied and Environmental Microbiology, May 2007, p. 3205-3214, Vol. 73, No. 10
0099-2240/07/$08.00+0     doi:10.1128/AEM.02985-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2007 by the American Society for Microbiology. All rights reserved.