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Applied and Environmental Microbiology, May 2007, p. 3232-3238, Vol. 73, No. 10
0099-2240/07/$08.00+0     doi:10.1128/AEM.02579-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Prevalence of Campylobacter spp. in Cattle in Finland and Antimicrobial Susceptibilities of Bovine Campylobacter jejuni Strains{triangledown}

Marjaana Hakkinen,1* Helmi Heiska,1 and Marja-Liisa Hänninen2

Finnish Food Safety Authority, Mustialankatu 3, Helsinki FI-00790, Finland,1 Department of Food and Environmental Hygiene, University of Helsinki, Helsinki FI-00014, Finland2

Received 9 November 2006/ Accepted 12 March 2007

The study investigated the prevalence of Campylobacter spp. in Finnish cattle at slaughter and carcass contamination after slaughter. During the period January to December 2003, bovine rectal fecal samples (n = 952) and carcass surface samples (n = 948) from 12 out of 15 Finnish slaughterhouses were examined. In total, campylobacters were detected in 31.1% of fecal samples and in 3.5% of carcass surface samples. Campylobacter jejuni was isolated from 19.5%, Campylobacter coli from 2.2%, and presumptive Campylobacter hyointestinalis from 10.8% of fecal samples. Campylobacters were detected in 4.4% and 37.4% of the fecal samples examined both by direct culture and by enrichment (n = 730), respectively, suggesting a low level of campylobacters in the intestinal content. A slightly increasing trend was observed in the overall prevalence of campylobacters towards the end of summer and autumn. Seventeen different serotypes were detected among the fecal C. jejuni isolates using a set of 25 commercial antisera for serotyping heat-stable antigens (Penner) of C. jejuni by passive hemagglutination. The predominant serotypes, Pen2 and Pen4-complex, were isolated from 52% of the fecal samples. Subtyping by pulsed-field gel electrophoresis (SmaI) yielded 56 and 20 subtypes out of 330 fecal and 70 carcass C. jejuni isolates, respectively. MICs of ampicillin, enrofloxacin, erythromycin, gentamicin, nalidixic acid, and oxytetracycline for 187 C. jejuni isolates were determined using a commercial broth microdilution method. Sixteen (9%) of the isolates were resistant to at least one of the antimicrobials tested. Resistance to nalidixic acid was most commonly detected (6%). No multiresistance was observed.

* Corresponding author. Mailing address: Finnish Food Safety Authority, Mustialankatu 3, Helsinki FI-00790, Finland. Phone: 358 2077 24471. Fax: 358 2077 24350. E-mail: marjaana.hakkinen{at}evira.fi

{triangledown} Published ahead of print on 16 March 2007.

Applied and Environmental Microbiology, May 2007, p. 3232-3238, Vol. 73, No. 10
0099-2240/07/$08.00+0     doi:10.1128/AEM.02579-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gonzalez, M., Hakkinen, M., Rautelin, H., Hanninen, M.-L. (2009). Bovine Campylobacter jejuni Strains Differ from Human and Chicken Strains in an Analysis of Certain Molecular Genetic Markers. Appl. Environ. Microbiol. 75: 1208-1210 [Abstract] [Full Text]  
  • Ragimbeau, C., Schneider, F., Losch, S., Even, J., Mossong, J. (2008). Multilocus Sequence Typing, Pulsed-Field Gel Electrophoresis, and fla Short Variable Region Typing of Clonal Complexes of Campylobacter jejuni Strains of Human, Bovine, and Poultry Origins in Luxembourg. Appl. Environ. Microbiol. 74: 7715-7722 [Abstract] [Full Text]  


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