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Applied and Environmental Microbiology, May 2007, p. 3431-3436, Vol. 73, No. 10
0099-2240/07/$08.00+0     doi:10.1128/AEM.02702-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

High-Resolution DNA Melt Curve Analysis of the Clustered, Regularly Interspaced Short-Palindromic-Repeat Locus of Campylobacter jejuni{triangledown} ,{dagger}

Erin P. Price,1 Helen Smith,2 Flavia Huygens,1 and Philip M. Giffard1*

Cooperative Research Centre for Diagnostics, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia,1 Queensland Health Scientific Services, Coopers Plains, Australia2

Received 20 November 2006/ Accepted 17 March 2007

A novel method for genotyping the clustered, regularly interspaced short-palindromic-repeat (CRISPR) locus of Campylobacter jejuni is described. Following real-time PCR, CRISPR products were subjected to high-resolution melt (HRM) analysis, a new technology that allows precise melt profile determination of amplicons. This investigation shows that the CRISPR HRM assay provides a powerful addition to existing C. jejuni genotyping methods and emphasizes the potential of HRM for genotyping short sequence repeats in other species.

* Corresponding author. Mailing address: Cooperative Research Centre for Diagnostics, Institute of Health and Biomedical Innovation, Queensland University of Technology, Cnr Blamey St. and Musk Ave., Kelvin Grove, Queensland 4059, Australia. Phone: 61 7 3138 6194. Fax: 61 7 3138 6030. E-mail: p.giffard{at}qut.edu.au

{triangledown} Published ahead of print on 30 March 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, May 2007, p. 3431-3436, Vol. 73, No. 10
0099-2240/07/$08.00+0     doi:10.1128/AEM.02702-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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