Previous Article | Next Article 
Applied and Environmental Microbiology, May 2007, p. 3431-3436, Vol. 73, No. 10
0099-2240/07/$08.00+0 doi:10.1128/AEM.02702-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.
High-Resolution DNA Melt Curve Analysis of the Clustered, Regularly Interspaced Short-Palindromic-Repeat Locus of Campylobacter jejuni
,
Erin P. Price,1
Helen Smith,2
Flavia Huygens,1 and
Philip M. Giffard1*
Cooperative Research Centre for Diagnostics, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia,1
Queensland Health Scientific Services, Coopers Plains, Australia2
Received 20 November 2006/
Accepted 17 March 2007
A novel method for genotyping the clustered, regularly interspaced short-palindromic-repeat (CRISPR) locus of Campylobacter jejuni is described. Following real-time PCR, CRISPR products were subjected to high-resolution melt (HRM) analysis, a new technology that allows precise melt profile determination of amplicons. This investigation shows that the CRISPR HRM assay provides a powerful addition to existing C. jejuni genotyping methods and emphasizes the potential of HRM for genotyping short sequence repeats in other species.
* Corresponding author. Mailing address: Cooperative Research Centre for Diagnostics, Institute of Health and Biomedical Innovation, Queensland University of Technology, Cnr Blamey St. and Musk Ave., Kelvin Grove, Queensland 4059, Australia. Phone: 61 7 3138 6194. Fax: 61 7 3138 6030. E-mail:
p.giffard{at}qut.edu.au
Published ahead of print on 30 March 2007.
Supplemental material for this article may be found at http://aem.asm.org/.
Applied and Environmental Microbiology, May 2007, p. 3431-3436, Vol. 73, No. 10
0099-2240/07/$08.00+0 doi:10.1128/AEM.02702-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
-
Cai, L, Kong, F, Toi, C, van Hal, S, Gilbert, G L
(2010). Differentiation of Chlamydia trachomatis lymphogranuloma venereum-related serovars from other serovars using multiplex allele-specific polymerase chain reaction and high-resolution melting analysis. Int J STD AIDS
21: 101-104
[Abstract]
[Full Text]
-
Merchant-Patel, S., Blackall, P. J., Templeton, J., Price, E. P., Tong, S. Y. C., Huygens, F., Giffard, P. M.
(2010). Campylobacter jejuni and Campylobacter coli Genotyping by High-Resolution Melting Analysis of a flaA Fragment. Appl. Environ. Microbiol.
76: 493-499
[Abstract]
[Full Text]
-
Steer, P. A., Kirkpatrick, N. C., O'Rourke, D., Noormohammadi, A. H.
(2009). Classification of Fowl Adenovirus Serotypes by Use of High-Resolution Melting-Curve Analysis of the Hexon Gene Region. J. Clin. Microbiol.
47: 311-321
[Abstract]
[Full Text]
-
Stephens, A. J., Inman-Bamber, J., Giffard, P. M., Huygens, F.
(2008). High-Resolution Melting Analysis of the spa Repeat Region of Staphylococcus aureus. Clin. Chem.
54: 432-436
[Abstract]
[Full Text]