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 Previous Article

Applied and Environmental Microbiology, May 2007, p. 3446-3449, Vol. 73, No. 10
0099-2240/07/$08.00+0     doi:10.1128/AEM.02478-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Differentiation of Bacillus anthracis, B. cereus, and B. thuringiensis by Using Pulsed-Field Gel Electrophoresis{triangledown}

Wenwan Zhong,2,{dagger} Yulin Shou,1 Thomas M. Yoshida,2 and Babetta L. Marrone1*

Bioscience,1 Chemistry Divisions, Los Alamos National Laboratory, Los Alamos, New Mexico 875452

Received 23 October 2006/ Accepted 18 March 2007

A pulsed-field gel electrophoresis (PFGE) method was developed for discriminating Bacillus anthracis from B. cereus and B. thuringiensis. A worldwide collection of 25 B. anthracis isolates showed high-profile homology, and these isolates were unambiguously distinguished from B. cereus and B. thuringiensis isolates by cluster analysis of the whole-genome macrorestriction enzyme digestion patterns generated by NotI.

* Corresponding author. Mailing address: Bioscience Division, MS M888, Los Alamos National Laboratory, Los Alamos, NM 87545. Phone: (505) 667-3279. Fax: (505) 665-3024. E-mail: blm{at}lanl.gov

{triangledown} Published ahead of print on 30 March 2007.

{dagger} Present address: Department of Chemistry, University of California, Riverside, Riverside, CA 92521.

Applied and Environmental Microbiology, May 2007, p. 3446-3449, Vol. 73, No. 10
0099-2240/07/$08.00+0     doi:10.1128/AEM.02478-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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Copyright © 2007 by the American Society for Microbiology. All rights reserved.