This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by DeAngelis, K. M.
Right arrow Articles by Lindow, S. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by DeAngelis, K. M.
Right arrow Articles by Lindow, S. E.
Agricola
Right arrow Articles by DeAngelis, K. M.
Right arrow Articles by Lindow, S. E.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, June 2007, p. 3724-3727, Vol. 73, No. 11
0099-2240/07/$08.00+0     doi:10.1128/AEM.02187-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Sensitive Whole-Cell Biosensor Suitable for Detecting a Variety of N-Acyl Homoserine Lactones in Intact Rhizosphere Microbial Communities{triangledown}

Kristen M. DeAngelis,1 Mary K. Firestone,2 and Steven E. Lindow1*

Department of Plant and Microbial Biology,1 Department of Environmental Science, Policy, and Management, University of California, Berkeley, California 947202

Received 17 September 2006/ Accepted 24 March 2007

To investigate quorum sensing in rhizosphere soil, a whole-cell biosensor, Agrobacterium tumefaciens(pAHL-Ice), was constructed. The biosensor responded to all N-acyl homoserine lactones (AHLs) tested, except C4 homoserine lactone, with a minimum detection limit of 10–12 M, as well as to both exogenously added AHLs and AHL-producing bacterial strains in soil. This highly sensitive biosensor reveals for the first time the increased AHL availability in intact rhizosphere microbial communities compared to that in bulk soil.

* Corresponding author. Mailing address: Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720. Phone: (510) 642-4174. Fax: (510) 642-4995. E-mail: icelab{at}socrates.berkeley.edu

{triangledown} Published ahead of print on 30 March 2007.

Applied and Environmental Microbiology, June 2007, p. 3724-3727, Vol. 73, No. 11
0099-2240/07/$08.00+0     doi:10.1128/AEM.02187-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»