AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
AEM.00057-07v1
73/11/3728    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Altic, L. C.
Right arrow Articles by Grant, I. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Altic, L. C.
Right arrow Articles by Grant, I. R.
Agricola
Right arrow Articles by Altic, L. C.
Right arrow Articles by Grant, I. R.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, June 2007, p. 3728-3733, Vol. 73, No. 11
0099-2240/07/$08.00+0     doi:10.1128/AEM.00057-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

UV Light Inactivation of Mycobacterium avium subsp. paratuberculosis in Milk as Assessed by FASTPlaqueTB Phage Assay and Culture{triangledown}

Leslie C. Altic,1 Michael T. Rowe,2 and Irene R. Grant1*

Institute of Agri-Food and Land Use, School of Biological Sciences, Queen's University Belfast,1 Agri-Food and Biosciences Institute for Northern Ireland, Belfast, Northern Ireland, United Kingdom2

Received 10 January 2007/ Accepted 4 April 2007

UV light inactivation of Mycobacterium avium subsp. paratuberculosis in Middlebrook 7H9 broth and whole and semiskim milk was investigated using a laboratory-scale UV machine that incorporated static mixers within UV-penetrable pipes. UV treatment proved to be less effective in killing M. avium subsp. paratuberculosis suspended in milk (0.5- to 1.0-log10 reduction per 1,000 mJ/ml) than that suspended in Middlebrook 7H9 broth (2.5- to 3.3-log10 reduction per 1,000 mJ/ml). The FASTPlaqueTB phage assay provided more rapid enumeration of surviving M. avium subsp. paratuberculosis (within 24 h) than culture on Herrold's egg yolk medium (6 to 8 weeks). Despite the fact that plaque counts were consistently 1 to 2 log10 lower than colony counts throughout the study, UV inactivation rates for M. avium subsp. paratuberculosis derived using the phage assay and culture results were not significantly different (P = 0.077).

* Corresponding author. Mailing address: Institute of Agri-Food and Land Use, School of Biological Sciences, Queen's University Belfast, David Keir Building, Stranmillis Road, Belfast BT9 5AG, United Kingdom. Phone: 44 28 9097 6561. Fax: 44 28 9097 6513. E-mail: i.grant{at}qub.ac.uk

{triangledown} Published ahead of print on 13 April 2007.

Applied and Environmental Microbiology, June 2007, p. 3728-3733, Vol. 73, No. 11
0099-2240/07/$08.00+0     doi:10.1128/AEM.00057-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2007 by the American Society for Microbiology. All rights reserved.