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Applied and Environmental Microbiology, June 2007, p. 3728-3733, Vol. 73, No. 11
0099-2240/07/$08.00+0     doi:10.1128/AEM.00057-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

UV Light Inactivation of Mycobacterium avium subsp. paratuberculosis in Milk as Assessed by FASTPlaqueTB Phage Assay and Culture{triangledown}

Leslie C. Altic,1 Michael T. Rowe,2 and Irene R. Grant1*

Institute of Agri-Food and Land Use, School of Biological Sciences, Queen's University Belfast,1 Agri-Food and Biosciences Institute for Northern Ireland, Belfast, Northern Ireland, United Kingdom2

Received 10 January 2007/ Accepted 4 April 2007

UV light inactivation of Mycobacterium avium subsp. paratuberculosis in Middlebrook 7H9 broth and whole and semiskim milk was investigated using a laboratory-scale UV machine that incorporated static mixers within UV-penetrable pipes. UV treatment proved to be less effective in killing M. avium subsp. paratuberculosis suspended in milk (0.5- to 1.0-log10 reduction per 1,000 mJ/ml) than that suspended in Middlebrook 7H9 broth (2.5- to 3.3-log10 reduction per 1,000 mJ/ml). The FASTPlaqueTB phage assay provided more rapid enumeration of surviving M. avium subsp. paratuberculosis (within 24 h) than culture on Herrold's egg yolk medium (6 to 8 weeks). Despite the fact that plaque counts were consistently 1 to 2 log10 lower than colony counts throughout the study, UV inactivation rates for M. avium subsp. paratuberculosis derived using the phage assay and culture results were not significantly different (P = 0.077).

* Corresponding author. Mailing address: Institute of Agri-Food and Land Use, School of Biological Sciences, Queen's University Belfast, David Keir Building, Stranmillis Road, Belfast BT9 5AG, United Kingdom. Phone: 44 28 9097 6561. Fax: 44 28 9097 6513. E-mail: i.grant{at}qub.ac.uk

{triangledown} Published ahead of print on 13 April 2007.

Applied and Environmental Microbiology, June 2007, p. 3728-3733, Vol. 73, No. 11
0099-2240/07/$08.00+0     doi:10.1128/AEM.00057-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Foddai, A., Elliott, C. T., Grant, I. R. (2009). Optimization of a Phage Amplification Assay To Permit Accurate Enumeration of Viable Mycobacterium avium subsp. paratuberculosis Cells. Appl. Environ. Microbiol. 75: 3896-3902 [Abstract] [Full Text]  
  • Wong, S. Y. Y., Grant, I. R., Friedman, M., Elliott, C. T., Situ, C. (2008). Antibacterial Activities of Naturally Occurring Compounds against Mycobacterium avium subsp. paratuberculosis. Appl. Environ. Microbiol. 74: 5986-5990 [Abstract] [Full Text]  


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