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Applied and Environmental Microbiology, June 2007, p. 3734-3737, Vol. 73, No. 11
0099-2240/07/$08.00+0     doi:10.1128/AEM.02204-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Development of High-Throughput Phenotyping of Metagenomic Clones from the Human Gut Microbiome for Modulation of Eukaryotic Cell Growth{triangledown}

Karine Gloux,1 Marion Leclerc,1 Harout Iliozer,1 René L'Haridon,2 Chaysavanh Manichanh,1 Gérard Corthier,1 Renaud Nalin,3 Hervé M. Blottière,1 and Joël Doré1*

Unité d'Ecologie et Physiologie du Système Digestif,1 Unité de Virologie et Immunologie Moléculaires, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas, France,2 LibraGen, Bat. Canal Biotech I, 3 rue des Satellites, 31400 Toulouse, France3

Received 19 September 2006/ Accepted 24 March 2007

Metagenomic libraries derived from human intestinal microbiota (20,725 clones) were screened for epithelial cell growth modulation. Modulatory clones belonging to the four phyla represented among the metagenomic libraries were identified (hit rate, 0.04 to 8.7% depending on the screening cutoff). Several candidate loci were identified by transposon mutagenesis and subcloning.

* Corresponding author. Mailing address: INRA, Unité d'Ecologie et de Physiologie du Système Digestif, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France. Phone: 33 (0)1 34 65 27 09. Fax: 33 (0)1 34 65 24 92. E-mail: joel.dore{at}jouy.inra.fr

{triangledown} Published ahead of print on 30 March 2007.

Applied and Environmental Microbiology, June 2007, p. 3734-3737, Vol. 73, No. 11
0099-2240/07/$08.00+0     doi:10.1128/AEM.02204-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2007 by the American Society for Microbiology. All rights reserved.