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Applied and Environmental Microbiology, June 2007, p. 3759-3764, Vol. 73, No. 11
0099-2240/07/$08.00+0     doi:10.1128/AEM.02185-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Purification, Characterization, Gene Cloning, and Expression of a Novel Alcohol Dehydrogenase with Anti-Prelog Stereospecificity from Candida parapsilosis{triangledown} ,{dagger}

Yao Nie, Yan Xu,* Xiao Qing Mu, Hai Yan Wang, Ming Yang, and Rong Xiao

Key Laboratory of Industrial Biotechnology of Ministry of Education and School of Biotechnology, Southern Yangtze University, Wuxi 214122, People's Republic of China

Received 16 September 2006/ Accepted 31 March 2007

An alcohol dehydrogenase from Candida parapsilosis CCTCC M203011 was characterized along with its biochemical activity and structural gene. The amino acid sequence shows similarity to those of the short-chain dehydrogenase/reductases but no overall identity to known proteins. This enzyme with unusual stereospecificity catalyzes an anti-Prelog reduction of 2-hydroxyacetophenone to (S)-1-phenyl-1,2-ethanediol.

* Corresponding author. Mailing address: Key Laboratory of Industrial Biotechnology of Ministry of Education and School of Biotechnology, Southern Yangtze University, 1800 Lihu Rd., Wuxi 214122, People's Republic of China. Phone: 86-510-85864735. Fax: 86-510-85864112. E-mail: yxu{at}sytu.edu.cn

{triangledown} Published ahead of print on 13 April 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, June 2007, p. 3759-3764, Vol. 73, No. 11
0099-2240/07/$08.00+0     doi:10.1128/AEM.02185-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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