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Applied and Environmental Microbiology, June 2007, p. 3845-3849, Vol. 73, No. 12
0099-2240/07/$08.00+0     doi:10.1128/AEM.00019-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Characterization of Staphylococcus aureus Isolates from Buffalo, Bovine, Ovine, and Caprine Milk Samples Collected in Rio de Janeiro State, Brazil{triangledown}

Marta Aires-de-Sousa,1 Carlos E. S. R. Parente,2 Olney Vieira-da-Motta,2 Isabel C. F. Bonna,2 Denise A. Silva,2 and Hermínia de Lencastre1,3*

Laboratório de Genética Molecular, Instituto de Tecnologia Química e Biológica da Universidade Nova de Lisboa (ITQB/UNL), Oeiras, Portugal,1 Laboratório de Sanidade Animal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense-UENF, Campos dos Goytacazes, Rio de Janeiro, Brazil,2 Laboratory of Microbiology, The Rockefeller University, New York, New York3

Received 10 December 2006/ Accepted 9 April 2007

Eighty-four staphylococcal isolates were obtained from milk samples from cows, sheep, goats, and buffalo with subclinical mastitis and from colonization samples from ostriches. The animals were hosted in 18 small dairy herds and an ostrich breeding located in 10 municipalities of the state of Rio de Janeiro, Brazil. Thirty isolates were identified as Staphylococcus aureus by biochemical and molecular techniques and were comparatively characterized by phenotypic and genotypic methods. The molecular characterization by pulsed-field gel electrophoresis (PFGE), spa typing, and multilocus sequence typing (MLST) revealed five clonal types (PFGE A, spa type t359, sequence type 747 [ST747]; PFGE B, spa type t1180, ST750; PFGE C, spa type t605, ST126; PFGE D, spa type t127, ST751; and PFGE F, spa type t002, ST5). None of the isolates harbored the Panton-Valentine leukocidin or exfoliative toxin D gene. The detection of major clone A (in 63% of the isolates) in different herds, among all animal species studied, and in infection and colonization samples evidenced its geographical spread among Rio de Janeiro State and no host preference among the animal species. Comparison with S. aureus from a human origin suggested that all but one clone found in the present study might be animal specific.

* Corresponding author. Mailing address: The Rockefeller University, 1230 York Ave., New York, NY 10021. Phone: (212) 327-8278. Fax: (212) 327-8688. E-mail: lencash{at}mail.rockefeller.edu

{triangledown} Published ahead of print on 20 April 2007.

Applied and Environmental Microbiology, June 2007, p. 3845-3849, Vol. 73, No. 12
0099-2240/07/$08.00+0     doi:10.1128/AEM.00019-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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