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Applied and Environmental Microbiology, June 2007, p. 3850-3858, Vol. 73, No. 12
0099-2240/07/$08.00+0     doi:10.1128/AEM.00243-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Identification of a Gene Cluster Enabling Lactobacillus casei BL23 To Utilize myo-Inositol{triangledown} ,{dagger}

María Jesús Yebra,1 Manuel Zúñiga,1 Sophie Beaufils,2 Gaspar Pérez-Martínez,1 Josef Deutscher,2 and Vicente Monedero1*

Laboratorio de Bacterias Lácticas y Probióticos, IATA-CSIC, P.O. Box 73, 46100 Burjassot, Valencia, Spain,1 Laboratoire de Microbiologie et Génétique Moleculaire, INRA-AgroParisTech-CNRS, F-78850 Thiverval-Grignon, France2

Received 31 January 2007/ Accepted 11 April 2007

Genome analysis of Lactobacillus casei BL23 revealed that, compared to L. casei ATCC 334, it carries a 12.8-kb DNA insertion containing genes involved in the catabolism of the cyclic polyol myo-inositol (MI). Indeed, L. casei ATCC 334 does not ferment MI, whereas strain BL23 is able to utilize this carbon source. The inserted DNA consists of an iolR gene encoding a DeoR family transcriptional repressor and a divergently transcribed iolTABCDG1G2EJK operon, encoding a complete MI catabolic pathway, in which the iolK gene probably codes for a malonate semialdehyde decarboxylase. The presence of iolK suggests that L. casei has two alternative pathways for the metabolism of malonic semialdehyde: (i) the classical MI catabolic pathway in which IolA (malonate semialdehyde dehydrogenase) catalyzes the formation of acetyl-coenzyme A from malonic semialdehyde and (ii) the conversion of malonic semialdehyde to acetaldehyde catalyzed by the product of iolK. The function of the iol genes was verified by the disruption of iolA, iolT, and iolD, which provided MI-negative strains. By contrast, the disruption of iolK resulted in a strain with no obvious defect in MI utilization. Transcriptional analyses conducted with different mutant strains showed that the iolTABCDG1G2EJK cluster is regulated by substrate-specific induction mediated by the inactivation of the transcriptional repressor IolR and by carbon catabolite repression mediated by the catabolite control protein A (CcpA). This is the first example of an operon for MI utilization in lactic acid bacteria and illustrates the versatility of carbohydrate utilization in L. casei BL23.

* Corresponding author. Mailing address: Laboratorio de Bacterias Lácticas y Probióticos, IATA-CSIC, P.O. Box 73, 46100 Burjassot, Valencia, Spain. Phone: 34 963900022. Fax: 34 963636301. E-mail: btcmon{at}iata.csic.es

{triangledown} Published ahead of print on 20 April 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, June 2007, p. 3850-3858, Vol. 73, No. 12
0099-2240/07/$08.00+0     doi:10.1128/AEM.00243-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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