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Applied and Environmental Microbiology, June 2007, p. 4082-4088, Vol. 73, No. 12
0099-2240/07/$08.00+0     doi:10.1128/AEM.01820-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

DNA Microarray-Based Identification of Serogroups and Virulence Gene Patterns of Escherichia coli Isolates Associated with Porcine Postweaning Diarrhea and Edema Disease ^,

Weiqing Han,^1,2,3 Bin Liu,^1,2,3 Boyang Cao,^1,2,3 Lothar Beutin,⁴ Ulrike Krüger,⁴ Hongbo Liu,^1,2,3 Yayue Li,^1,2,3 Yanqun Liu,^1,2,3 Lu Feng,^1,2,3 and Lei Wang^1,2,3*

TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin 300457, China,¹ Tianjin Key Laboratory of Microbial Functional Genomics, 23 Hongda Street, TEDA, Tianjin 300457, China,² Tianjin Research Center for Functional Genomics and Biochip, 23 Hongda Street, TEDA, Tianjin 300457, China,³ National Reference Laboratory for Escherichia coli, Federal Institute for Risk Assessment (BfR), Diedersdorfer Weg 1, D-12277 Berlin, Germany⁴

Received 2 August 2006/ Accepted 13 April 2007

Escherichia coli strains causing postweaning diarrhea (PWD) and edema disease (ED) in pigs are limited to a number of serogroups, with O8, O45, O138, O139, O141, O147, O149, and O157 being the most commonly reported worldwide. In this study, a DNA microarray based on the O-antigen-specific genes of all 8 E. coli serogroups, as well as 11 genes encoding adhesion factors and exotoxins associated with PWD and ED, was developed for the identification of related serogroups and virulence gene patterns. The microarray method was tested against 186 E. coli and Shigella O-serogroup reference strains, 13 E. coli reference strains for virulence markers, 43 E. coli clinical isolates, and 12 strains of other bacterial species and shown to be highly specific with reproducible results. The detection sensitivity was 0.1 ng of genomic DNA or 10³ CFU per 0.3 g of porcine feces in mock samples. Seventeen porcine feces samples from local hoggeries were examined using the microarray, and the result for one sample was verified by the conventional serotyping methods. This microarray can be readily used to screen for the presence of PWD- and ED-associated E. coli in porcine feces samples.

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* Corresponding author. Mailing address: TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin 300457, China. Phone: 86-22-66229588. Fax: 86-22-66229596. E-mail: wanglei{at}nankai.edu.cn

Published ahead of print on 20 April 2007.

Supplemental material for this article may be found at http://aem.asm.org/.

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Applied and Environmental Microbiology, June 2007, p. 4082-4088, Vol. 73, No. 12
0099-2240/07/$08.00+0     doi:10.1128/AEM.01820-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

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