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Applied and Environmental Microbiology, July 2007, p. 4180-4184, Vol. 73, No. 13
0099-2240/07/$08.00+0     doi:10.1128/AEM.02225-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Occurrence of Antibiotic-Resistant Uropathogenic Escherichia coli Clonal Group A in Wastewater Effluents{triangledown}

Laura A. Boczek,1 Eugene W. Rice,1 Brian Johnston,2 and James R. Johnson2*

U.S. Environmental Protection Agency, Cincinnati, Ohio,1 Medical Service, Veterans Affairs Medical Center, and Department of Medicine, University of Minnesota, Minneapolis, Minnesota2

Received 21 September 2006/ Accepted 27 April 2007

Isolates of Escherichia coli belonging to clonal group A (CGA), a recently described disseminated cause of drug-resistant urinary tract infections in humans, were present in four of seven sewage effluents collected from geographically dispersed areas of the United States. All 15 CGA isolates (1% of the 1,484 isolates analyzed) exhibited resistance to trimethoprim-sulfamethoxazole (TMP-SMZ), accounting for 19.5% of the 77 TMP-SMZ-resistant isolates. Antimicrobial resistance patterns, virulence traits, O:H serotypes, and phylogenetic groupings were compared for CGA and selected non-CGA isolates. The CGA isolates exhibited a wider diversity of resistance profiles and somatic antigens than that found in most previous characterizations of this clonal group. This is the first report of recovery from outside a human host of E. coli CGA isolates with virulence factor and antibiotic resistance profiles typical of CGA isolates from a human source. The occurrence of "human-type" CGA in wastewater effluents demonstrates a potential mode for the dissemination of this clonal group in the environment, with possible secondary transmission to new human or animal hosts.

* Corresponding author. Mailing address: Infectious Diseases (111F), Minneapolis VA Medical Center, 1 Veterans Drive, Minneapolis, MN 55417. Phone: (612) 467-4185. Fax: (612) 727-5995. E-mail: johns007{at}umn.edu

{triangledown} Published ahead of print on 4 May 2007.

Applied and Environmental Microbiology, July 2007, p. 4180-4184, Vol. 73, No. 13
0099-2240/07/$08.00+0     doi:10.1128/AEM.02225-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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