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Applied and Environmental Microbiology, July 2007, p. 4359-4364, Vol. 73, No. 13
0099-2240/07/$08.00+0     doi:10.1128/AEM.00258-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Use of Selective Capture of Transcribed Sequences To Identify Genes Preferentially Expressed by Streptococcus suis upon Interaction with Porcine Brain Microvascular Endothelial Cells{triangledown}

Nahuel Fittipaldi,1,2 Marcelo Gottschalk,1,2 Ghyslaine Vanier,1,2 France Daigle,1,3 and Josée Harel1,2*

Groupe de Recherche sur les Maladies Infectieuses du Porc and Centre de Recherche en Infectiologie Porcine,1 Faculté de Médecine Vétérinaire, Université de Montréal, St-Hyacinthe, Quebec J2S 7C6, Canada,2 Département de Microbiologie et Immunologie, Université de Montréal, Montreal, Quebec H3C 3J7, Canada3

Received 31 January 2007/ Accepted 29 April 2007

By using the selective capture of transcribed sequences (SCOTS) approach, we identified 28 genes preferentially expressed by the major swine pathogen and zoonotic agent Streptococcus suis upon interaction with porcine brain microvascular endothelial cells. Several of these genes may be considered new S. suis candidate virulence factors. Results from this study demonstrate the suitability of SCOTS for the elucidation of gene expression in streptococcal species and may contribute to a better understanding of the pathogenesis of S. suis infections.

* Corresponding author. Mailing address: GREMIP, Faculté de Médecine Vétérinaire, Université de Montréal, CP 5000, St-Hyacinthe, Quebec J2S 7C6, Canada. Phone: (450) 773-8521, ext. 1-8233. Fax: (450) 778-8108. E-mail: josee.harel{at}umontreal.ca

{triangledown} Published ahead of print on 4 May 2007.

Applied and Environmental Microbiology, July 2007, p. 4359-4364, Vol. 73, No. 13
0099-2240/07/$08.00+0     doi:10.1128/AEM.00258-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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