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Applied and Environmental Microbiology, July 2007, p. 4368-4372, Vol. 73, No. 13
0099-2240/07/$08.00+0     doi:10.1128/AEM.00086-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Identification of Polypeptides Expressed in Response to Vinyl Chloride, Ethene, and Epoxyethane in Nocardioides sp. Strain JS614 by Using Peptide Mass Fingerprinting{triangledown} ,{dagger}

Adina S. Chuang and Timothy E. Mattes*

Department of Civil and Environmental Engineering, 4105 Seamans Center, University of Iowa, Iowa City, Iowa 52242

Received 13 January 2007/ Accepted 30 April 2007

Enzymes expressed in response to vinyl chloride, ethene, and epoxyethane by Nocardioides sp. strain JS614 were identified by using a peptide mass fingerprinting (PMF) approach. PMF provided insight concerning vinyl chloride biodegradation in strain JS614 and extends the use of matrix-assisted laser desorption-ionization time of flight mass spectrometry as a tool to enhance characterization of biodegradation pathways.

* Corresponding author. Mailing address: Department of Civil and Environmental Engineering, 4105 Seamans Center, University of Iowa, Iowa City, IA 52242. Phone: (319) 335-5065. Fax: (319) 335-5660. E-mail: tim-mattes{at}uiowa.edu

{triangledown} Published ahead of print on 4 May 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, July 2007, p. 4368-4372, Vol. 73, No. 13
0099-2240/07/$08.00+0     doi:10.1128/AEM.00086-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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