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Applied and Environmental Microbiology, July 2007, p. 4373-4378, Vol. 73, No. 13
0099-2240/07/$08.00+0     doi:10.1128/AEM.00271-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Pichia acaciae Killer System: Genetic Analysis of Toxin Immunity{triangledown}

John P. Paluszynski, Roland Klassen, and Friedhelm Meinhardt*

Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstr. 3, D-48149 Münster, Germany

Received 2 February 2007/ Accepted 28 April 2007

The gene responsible for self-protection in the Pichia acaciae killer plasmid system was identified by heterologous expression in Saccharomyces cerevisiae. Resistance profiling and conditional toxin/immunity coexpression analysis revealed dose-independent protection by pPac1-2 ORF4 and intracellular interference with toxin function, suggesting toxin reinternalization in immune killer cells.

* Corresponding author. Mailing address: Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstr. 3, D-48149 Münster, Germany. Phone: 49 2 51 83-3 98 25. Fax: 49 2 51 83-3 83 88. E-mail: meinhar{at}uni-muenster.de

{triangledown} Published ahead of print on 4 May 2007.

Applied and Environmental Microbiology, July 2007, p. 4373-4378, Vol. 73, No. 13
0099-2240/07/$08.00+0     doi:10.1128/AEM.00271-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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