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Applied and Environmental Microbiology, July 2007, p. 4385-4388, Vol. 73, No. 13
0099-2240/07/$08.00+0     doi:10.1128/AEM.02470-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Screening of Exopolysaccharide-Producing Lactobacillus and Bifidobacterium Strains Isolated from the Human Intestinal Microbiota{triangledown}

Patricia Ruas-Madiedo,* José Antonio Moreno,{dagger}, Nuria Salazar, Susana Delgado, Baltasar Mayo, Abelardo Margolles, and Clara G. de los Reyes-Gavilán

Departamento de Microbiologia Bioquímica de Productos Lácteos, Instituto de Productos Lácteos de Asturias, CSIC, Carretera de Infiesto s/n, 33300 Villaviciosa, Asturias, Spain

Received 23 October 2006/ Accepted 26 April 2007

Using phenotypic approaches, we have detected that 17% of human intestinal Lactobacillus and Bifidobacterium strains could be exopolysaccharide (EPS) producers. However, PCR techniques showed that only 7% harbored genes related to the synthesis of heteropolysaccharides. This is the first work to screen the human intestinal ecosystem for the detection of EPS-producing strains.

* Corresponding author. Mailing address: Instituto de Productos Lácteos de Asturias, CSIC, Ctra. Infiesto s/n (P.O. 85), 33300 Villaviciosa (Asturias), Spain. Phone: 34-985892131. Fax: 34-985892233. E-mail: ruas-madiedo{at}ipla.csic.es

{triangledown} Published ahead of print on 4 May 2007.

{dagger} Present address: Laboratorios Ordesa, Carretera del Prat 9-11, 08830 Sant Boi Llobregat, Spain.

Applied and Environmental Microbiology, July 2007, p. 4385-4388, Vol. 73, No. 13
0099-2240/07/$08.00+0     doi:10.1128/AEM.02470-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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