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Applied and Environmental Microbiology, July 2007, p. 4673-4676, Vol. 73, No. 14
0099-2240/07/$08.00+0     doi:10.1128/AEM.02891-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Mixed-Species Biofilm Formation by Lactic Acid Bacteria and Rice Wine Yeasts{triangledown}

Taketo Kawarai,1 Soichi Furukawa,1 Hirokazu Ogihara,1 and Makari Yamasaki2*

Department of Food Science and Technology, College of Bioresource Sciences,1 Advanced Research Institute for the Sciences and Humanities, Nihon University, Tokyo, Japan2

Received 14 December 2006/ Accepted 15 May 2007

We found that species combinations such as Lactobacillus casei subsp. rhamnosus IFO3831 and Saccharomyces cerevisiae Kyokai-10 can form a mixed-species biofilm in coculture. Moreover, the Kyokai-10 yeast strain can form a biofilm in monoculture in the presence of conditioned medium (CM) from L. casei IFO3831. The active substance(s) in bacterial CM is heat sensitive and has a molecular mass of between 3 and 5 kDa. In biofilms from cocultures or CM monocultures, yeast cells had a distinct morphology, with many hill-like protrusions on the cell surface.

* Corresponding author. Mailing address: Advanced Research Institute for the Sciences and Humanities, Nihon University, 6F, Ichigaya Tokyu Building, 4-2-1 Kudan-kita, Chiyoda-ku, Tokyo 102-0073, Japan. Phone: 81-3-5275-7954. Fax: 81-3-5275-7852. E-mail: yamasaki-makari{at}arish.nihon-u.ac.jp

{triangledown} Published ahead of print on 25 May 2007.

Applied and Environmental Microbiology, July 2007, p. 4673-4676, Vol. 73, No. 14
0099-2240/07/$08.00+0     doi:10.1128/AEM.02891-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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