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 Previous Article

Applied and Environmental Microbiology, July 2007, p. 4704-4706, Vol. 73, No. 14
0099-2240/07/$08.00+0     doi:10.1128/AEM.02807-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Luciferase Detection during Stationary Phase in Lactococcus lactis{triangledown}

Herwig Bachmann,1 Filipe Santos,1,2 Michiel Kleerebezem,1,2 and Johan E. T. van Hylckama Vlieg1,2*

NIZO food research, Kluyver Centre for Genomics of Industrial Fermentation, P.O. Box 20, 6710 BA Ede, The Netherlands,1 TI Food & Nutrition, P.O. Box 557,6700 AN Wageningen, The Netherlands2

Received 1 December 2006/ Accepted 15 May 2007

The luminescence signal of luxAB-encoded bacterial luciferase strongly depends on the metabolic state of the host cell, which restricts the use of this reporter system to metabolically active bacteria. Here we show that in stationary-phase cells of Lactococcus lactis, detection of luciferase is significantly improved by the addition of riboflavin or flavin mononucleotide to whole-cell assay systems.

* Corresponding author. Mailing address: NIZO food research, P.O. Box 20, 6710 BA Ede, The Netherlands. Phone: 31-318659511. Fax: 31-318650400. E-mail: johan.van.hylckama.vlieg{at}nizo.nl

{triangledown} Published ahead of print on 18 May 2007.

Applied and Environmental Microbiology, July 2007, p. 4704-4706, Vol. 73, No. 14
0099-2240/07/$08.00+0     doi:10.1128/AEM.02807-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Rodriguez-Garcia, A., Sola-Landa, A., Apel, K., Santos-Beneit, F., Martin, J. F. (2009). Phosphate control over nitrogen metabolism in Streptomyces coelicolor: direct and indirect negative control of glnR, glnA, glnII and amtB expression by the response regulator PhoP. Nucleic Acids Res 37: 3230-3242 [Abstract] [Full Text]  
  • Bachmann, H., Kleerebezem, M., van Hylckama Vlieg, J. E. T. (2008). High-Throughput Identification and Validation of In Situ-Expressed Genes of Lactococcus lactis. Appl. Environ. Microbiol. 74: 4727-4736 [Abstract] [Full Text]  


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