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Applied and Environmental Microbiology, August 2007, p. 5026-5033, Vol. 73, No. 15
0099-2240/07/$08.00+0     doi:10.1128/AEM.00600-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

NCgl2620 Encodes a Class II Polyphosphate Kinase in Corynebacterium glutamicum{triangledown}

Steffen N. Lindner,1,2,{dagger} Dolores Vidaurre,2,{dagger} Sabine Willbold,3 Siegfried M. Schoberth,2 and Volker F. Wendisch1*

Institute of Molecular Microbiology and Biotechnology, Westfalian Wilhelms University Muenster, Muenster, Germany,1 Institut für Biotechnologie 1,2 Zentralabteilung für Chemische Analysen, Forschungszentrum Jülich, Jülich, Germany3

Received 15 March 2007/ Accepted 26 May 2007

Corynebacterium glutamicum is able to accumulate up to 600 mM cytosolic phosphorus in the form of polyphosphate (poly P). Granular poly P (volutin) can make up to 37% of the internal cell volume. This bacterium lacks the classic enzyme of poly P synthesis, class I polyphosphate kinase (PPK1), but it possesses two genes, ppk2A (corresponds to NCgl0880) and ppk2B (corresponds to NCgl2620), for putative class II (PPK2) PPKs. Deletion of ppk2B decreased PPK activity and cellular poly P content, while overexpression of ppk2B increased both PPK activity and cellular poly P content. Neither deletion nor overexpression of ppk2A changed specific activity of PPK or cellular poly P content significantly. Purified PPK2B of C. glutamicum is active as a homotetramer and formed poly P with an average chain length of about 125, as determined with 31P nuclear magnetic resonance. The catalytic efficiency of C. glutamicum PPK2B was higher in the poly P-forming direction than for nucleoside triphosphate formation from poly P. The ppk2B deletion mutant, which accumulated very little poly P and grew as C. glutamicum wild type under phosphate-sufficient conditions, showed a growth defect under phosphate-limiting conditions.

* Corresponding author. Mailing address: Institute of Molecular Microbiology and Biotechnology, Westfalian Wilhelms University Muenster, Correnstr. 3, D-48149 Muenster, Germany. Phone: 49-251-833 9827. Fax: 49-251-833 8388. E-mail: wendisch{at}uni-muenster.de

{triangledown} Published ahead of print on 1 June 2007.

{dagger} These authors were equal contributors.

Applied and Environmental Microbiology, August 2007, p. 5026-5033, Vol. 73, No. 15
0099-2240/07/$08.00+0     doi:10.1128/AEM.00600-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lindner, S. N., Knebel, S., Wesseling, H., Schoberth, S. M., Wendisch, V. F. (2009). Exopolyphosphatases PPX1 and PPX2 from Corynebacterium glutamicum. Appl. Environ. Microbiol. 75: 3161-3170 [Abstract] [Full Text]  
  • Seufferheld, M. J., Alvarez, H. M., Farias, M. E. (2008). Role of Polyphosphates in Microbial Adaptation to Extreme Environments. Appl. Environ. Microbiol. 74: 5867-5874 [Full Text]  


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