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Applied and Environmental Microbiology, August 2007, p. 5048-5051, Vol. 73, No. 15
0099-2240/07/$08.00+0     doi:10.1128/AEM.02973-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Self-Formed Adaptor PCR: a Simple and Efficient Method for Chromosome Walking{triangledown} ,{dagger}

Shiming Wang,1,2 Jian He,1 Zhongli Cui,1 and Shunpeng Li1*

Key Laboratory of Microbiological Engineering of Agricultural Environment, Department of Microbiology, Nanjing Agricultural University, Nanjing, People's Republic of China,1 Department of Bioengineering, School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing, People's Republic of China2

Received 22 December 2006/ Accepted 1 May 2007

We developed a self-formed adaptor PCR (termed SEFA PCR) which can be used for chromosome walking. Most of the amplified flanking sequences were longer than 2.0 kb, and some were as long as 6.0 kb. SEFA PCR is simple and efficient and should have broad applications in the isolation of unknown sequences in complex genomes.

* Corresponding author. Mailing address: Department of Microbiology, Nanjing Agricultural University, No. 1 Weigang, Nanjing 210095, People's Republic of China. Phone and fax: 011-86-25-84396314. E-mail: lsp{at}njau.edu.cn

{triangledown} Published ahead of print on 4 May 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, August 2007, p. 5048-5051, Vol. 73, No. 15
0099-2240/07/$08.00+0     doi:10.1128/AEM.02973-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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