This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by de María, N.
Right arrow Articles by Fernández-Pascual, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by de María, N.
Right arrow Articles by Fernández-Pascual, M.
Agricola
Right arrow Articles by de María, N.
Right arrow Articles by Fernández-Pascual, M.

Next Article 

Applied and Environmental Microbiology, August 2007, p. 5075-5082, Vol. 73, No. 16
0099-2240/07/$08.00+0     doi:10.1128/AEM.00392-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Putative Porin of Bradyrhizobium sp. (Lupinus) Bacteroids Induced by Glyphosate{triangledown}

Nuria de María,1,{dagger} Ángeles Guevara,2,{dagger} M. Teresa Serra,2 Isabel García-Luque,2 Alfonso González-Sama,1 Mario García de Lacoba,2 M. Rosario de Felipe,1 and Mercedes Fernández-Pascual1*

Instituto de Recursos Naturales, Centro de Ciencias Medioambientales, CSIC, Serrano, 115-bis, E-28006 Madrid, Spain,1 Centro de Investigaciones Biológicas, CSIC, Ramiro de Maéztu 9, 28040 Madrid, Spain2

Received 19 February 2007/ Accepted 30 May 2007

Application of glyphosate (N-[phosphonomethyl] glycine) to Bradyrhizobium sp. (Lupinus)-nodulated lupin plants caused modifications in the protein pattern of bacteroids. The most significant change was the presence of a 44-kDa polypeptide in bacteroids from plants treated with the higher doses of glyphosate employed (5 and 10 mM). The polypeptide has been characterized by the amino acid sequencing of its N terminus and the isolation and nucleic acid sequencing of its encoding gene. It is putatively encoded by a single gene, and the protein has been identified as a putative porin. Protein modeling revealed the existence of several domains sharing similarity to different porins, such as a transmembrane beta-barrel. The protein has been designated BLpp, for Bradyrhizobium sp. (Lupinus) putative porin, and would be the first porin described in Bradyrhizobium sp. (Lupinus). In addition, a putative conserved domain of porins has been identified which consists of 87 amino acids, located in the BLpp sequence 30 amino acids downstream of the N-terminal region. In bacteroids, mRNA of the BLpp gene shows a basal constitutive expression that increases under glyphosate treatment, and the expression of the gene is seemingly regulated at the transcriptional level. By contrast, in free-living bacteria glyphosate treatment leads to an inhibition of BLpp mRNA accumulation, indicating a different effect of glyphosate on BLpp gene expression in bacteroids and free-living bacteria. The possible role of BLpp in a metabolite interchange between Bradyrhizobium and lupin is discussed.

* Corresponding author. Mailing address: Centro de Ciencias Medioambientales, CSIC, Serrano, 115-bis, E-28006 Madrid, Spain. Phone: 34 91 7452500. Fax: 34 91 5640800. E-mail: mfernandezp{at}ccma.csic.es

{triangledown} Published ahead of print on 8 June 2007.

{dagger} Present address: Department of Forest Systems and Resources, Center of Forest Research, Carretera de La Coruña km 7.5, E-28040 Madrid, Spain.

Applied and Environmental Microbiology, August 2007, p. 5075-5082, Vol. 73, No. 16
0099-2240/07/$08.00+0     doi:10.1128/AEM.00392-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Vera, M., Pagliai, F., Guiliani, N., Jerez, C. A. (2008). The Chemolithoautotroph Acidithiobacillus ferrooxidans Can Survive under Phosphate-Limiting Conditions by Expressing a C-P Lyase Operon That Allows It To Grow on Phosphonates. Appl. Environ. Microbiol. 74: 1829-1835 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»