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Applied and Environmental Microbiology, August 2007, p. 5374-5377, Vol. 73, No. 16
0099-2240/07/$08.00+0     doi:10.1128/AEM.00722-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Recombinant Expression and Characterization of a Reducing-End Xylose-Releasing Exo-Oligoxylanase from Bifidobacterium adolescentis{triangledown} ,{dagger}

Stijn Lagaert,1 Steven Van Campenhout,1,2 Annick Pollet,2 Tine M. Bourgois,1* Jan A. Delcour,2 Christophe M. Courtin,2 and Guido Volckaert1

Division of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, B-3001 Leuven, Belgium,1 Laboratory of Food Chemistry and Biochemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B-3001 Leuven, Belgium2

Received 30 March 2007/ Accepted 9 June 2007

The family 8 glycoside hydrolase (RexA) from Bifidobacterium adolescentis was expressed in Escherichia coli. The recombinant enzyme was characterized as a reducing-end xylose-releasing exo-oligoxylanase. Apart from giving insights into this new class of enzymes, knowledge of the RexA enzyme helps to postulate a mechanism for the B. adolescentis breakdown of prebiotic xylooligosaccharides.

* Corresponding author. Mailing address: Katholieke Universiteit Leuven, Division of Gene Technology, Kasteelpark Arenberg 21, B-3001 Leuven, Belgium. Phone: 32 16 329673. Fax: 32 16 321965. E-mail: tine.bourgois{at}biw.kuleuven.be

{triangledown} Published ahead of print on 22 June 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, August 2007, p. 5374-5377, Vol. 73, No. 16
0099-2240/07/$08.00+0     doi:10.1128/AEM.00722-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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