AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
AEM.00721-07v1
73/17/5587    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ioos, R.
Right arrow Articles by Frey, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ioos, R.
Right arrow Articles by Frey, P.
Agricola
Right arrow Articles by Ioos, R.
Right arrow Articles by Frey, P.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, September 2007, p. 5587-5597, Vol. 73, No. 17
0099-2240/07/$08.00+0     doi:10.1128/AEM.00721-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Distribution and Expression of Elicitin Genes in the Interspecific Hybrid Oomycete Phytophthora alni{triangledown}

Renaud Ioos,1,2 Franck Panabières,3 Benoît Industri,3 Axelle Andrieux,1 and Pascal Frey1*

INRA, Nancy-Université, UMR1136, Interactions Arbres-Microorganismes, IFR 110, F-54280 Champenoux,1 Laboratoire National de la Protection des Végétaux, Unité de Mycologie Agricole et Forestière, IFR 110, Domaine de Pixérécourt, F-54220 Malzéville,2 INRA, UMR1064, Interactions Plantes-Microorganismes et Santé Végétale, F-06930 Sophia Antipolis Cedex, France3

Received 30 March 2007/ Accepted 20 June 2007

Phytophthora alni subsp. alni, P. alni subsp. multiformis, and P. alni subsp. uniformis are responsible for alder disease in Europe. Class I and II elicitin gene patterns of P. alni subsp. alni, P. alni subsp. multiformis, P. alni subsp. uniformis, and the phylogenetically close species P. cambivora and P. fragariae were studied through mRNA sequencing and 3' untranslated region (3'UTR)-specific PCRs and sequencing. The occurrence of multiple 3'UTR sequences in association with identical elicitin-encoding sequences in P. alni subsp. alni indicated duplication/recombination events. The mRNA pattern displayed by P. alni subsp. alni demonstrated that elicitin genes from all the parental genomes are actually expressed in this allopolyploid taxon. The complementary elicitin patterns resolved confirmed the possible involvement of P. alni subsp. multiformis and P. alni subsp. uniformis in the genesis of the hybrid species P. alni subsp. alni. The occurrence of multiple and common elicitin gene sequences throughout P. cambivora, P. fragariae, and P. alni sensu lato, not observed in other Phytophthora species, suggests that duplication of these genes occurred before the radiation of these species.

* Corresponding author. Mailing address: INRA, Nancy-Université, UMR1136, Interactions Arbres-Microorganismes, F-54280 Champenoux, France. Phone: 33 383 394056. Fax: 33 383 394069. E-mail: frey{at}nancy.inra.fr

{triangledown} Published ahead of print on 29 June 2007.

Applied and Environmental Microbiology, September 2007, p. 5587-5597, Vol. 73, No. 17
0099-2240/07/$08.00+0     doi:10.1128/AEM.00721-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2007 by the American Society for Microbiology. All rights reserved.