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Applied and Environmental Microbiology, September 2007, p. 5587-5597, Vol. 73, No. 17
0099-2240/07/$08.00+0     doi:10.1128/AEM.00721-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Distribution and Expression of Elicitin Genes in the Interspecific Hybrid Oomycete Phytophthora alni{triangledown}

Renaud Ioos,1,2 Franck Panabières,3 Benoît Industri,3 Axelle Andrieux,1 and Pascal Frey1*

INRA, Nancy-Université, UMR1136, Interactions Arbres-Microorganismes, IFR 110, F-54280 Champenoux,1 Laboratoire National de la Protection des Végétaux, Unité de Mycologie Agricole et Forestière, IFR 110, Domaine de Pixérécourt, F-54220 Malzéville,2 INRA, UMR1064, Interactions Plantes-Microorganismes et Santé Végétale, F-06930 Sophia Antipolis Cedex, France3

Received 30 March 2007/ Accepted 20 June 2007

Phytophthora alni subsp. alni, P. alni subsp. multiformis, and P. alni subsp. uniformis are responsible for alder disease in Europe. Class I and II elicitin gene patterns of P. alni subsp. alni, P. alni subsp. multiformis, P. alni subsp. uniformis, and the phylogenetically close species P. cambivora and P. fragariae were studied through mRNA sequencing and 3' untranslated region (3'UTR)-specific PCRs and sequencing. The occurrence of multiple 3'UTR sequences in association with identical elicitin-encoding sequences in P. alni subsp. alni indicated duplication/recombination events. The mRNA pattern displayed by P. alni subsp. alni demonstrated that elicitin genes from all the parental genomes are actually expressed in this allopolyploid taxon. The complementary elicitin patterns resolved confirmed the possible involvement of P. alni subsp. multiformis and P. alni subsp. uniformis in the genesis of the hybrid species P. alni subsp. alni. The occurrence of multiple and common elicitin gene sequences throughout P. cambivora, P. fragariae, and P. alni sensu lato, not observed in other Phytophthora species, suggests that duplication of these genes occurred before the radiation of these species.

* Corresponding author. Mailing address: INRA, Nancy-Université, UMR1136, Interactions Arbres-Microorganismes, F-54280 Champenoux, France. Phone: 33 383 394056. Fax: 33 383 394069. E-mail: frey{at}nancy.inra.fr

{triangledown} Published ahead of print on 29 June 2007.

Applied and Environmental Microbiology, September 2007, p. 5587-5597, Vol. 73, No. 17
0099-2240/07/$08.00+0     doi:10.1128/AEM.00721-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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