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Applied and Environmental Microbiology, September 2007, p. 5653-5656, Vol. 73, No. 17
0099-2240/07/$08.00+0 doi:10.1128/AEM.00667-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.
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Biomedical Proteomics Research Group, Department of Structural Biology and Bioinformatics, University of Geneva, 1 Rue Michel Servet, 1211 Geneva 14, Switzerland,1 Proteome Informatics Group, Swiss Institute of Bioinformatics, Geneva, Switzerland,2 Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (CSIC), Ctra. Infiesto s/n, 33300 Villaviciosa, Asturias, Spain3
Received 23 March 2007/ Accepted 25 June 2007
Stable isotope labeling of amino acids in cell culture was used for Bifidobacterium longum. A comprehensive proteomic strategy was developed and validated by designing an appropriate semidefined medium that allows stable replacement of natural leucine by [13C6]leucine. Using this strategy, proteins having variations of at least 50% in their expression rates can be quantified with great confidence.
Published ahead of print on 29 June 2007.
Supplemental material for this article may be found at http://aem.asm.org/.
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