AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
AEM.01152-07v1
73/17/5660    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Karanis, P.
Right arrow Articles by Inoue, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Karanis, P.
Right arrow Articles by Inoue, N.
Agricola
Right arrow Articles by Karanis, P.
Right arrow Articles by Inoue, N.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, September 2007, p. 5660-5662, Vol. 73, No. 17
0099-2240/07/$08.00+0     doi:10.1128/AEM.01152-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Development and Preliminary Evaluation of a Loop-Mediated Isothermal Amplification Procedure for Sensitive Detection of Cryptosporidium Oocysts in Fecal and Water Samples{triangledown}

Panagiotis Karanis,1,2* Oriel Thekisoe,1 Klytaimnistra Kiouptsi,1,2 Jerry Ongerth,3 Ikuo Igarashi,1 and Noborou Inoue1

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan,1 University of Cologne, Center of Anatomy, Institute II, Molecular and Medical Parasitology, Cologne, Germany,2 Civil Mining and Environmental Engineering, The University of Wollongong, Wollongong, NSW 2522, Australia3

Received 23 May 2007/ Accepted 26 June 2007

A loop-mediated isothermal amplification (LAMP) procedure for the detection of Cryptosporidium in environmental and fecal samples was developed and evaluated. This is the first demonstration of LAMP applied to detection of Cryptosporidium. Due to its specificity and simplicity, the method could become a useful diagnostic tool for epidemiologic studies of Cryptosporidium presence.

* Corresponding author. Mailing address: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, Hokkaido 080-8555, Japan. Phone: (81) 155 49 5644. Fax: (81) 155 49 5643. E-mail: Panagiotis.Karanis{at}uk-koeln.de

{triangledown} Published ahead of print on 6 July 2007.

Applied and Environmental Microbiology, September 2007, p. 5660-5662, Vol. 73, No. 17
0099-2240/07/$08.00+0     doi:10.1128/AEM.01152-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2007 by the American Society for Microbiology. All rights reserved.