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Applied and Environmental Microbiology, September 2007, p. 5676-5678, Vol. 73, No. 17
0099-2240/07/$08.00+0     doi:10.1128/AEM.00278-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Use of an Escherichia coli Recombinant Producing Thermostable Polyphosphate Kinase as an ATP Regenerator To Produce Fructose 1,6-Diphosphate{triangledown} ,{dagger}

Seishi Iwamoto,1 Kei Motomura,1 Yasuharu Shinoda,1 Masaaki Urata,1 Junichi Kato,1 Noboru Takiguchi,1 Hisao Ohtake,2 Ryuichi Hirota,1 and Akio Kuroda1*

Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8530, Japan,1 Department of Biotechnology, Osaka University, Suita, Osaka 565-0871, Japan2

Received 3 February 2007/ Accepted 16 June 2007

Heat-treated Escherichia coli producing Thermus polyphosphate kinase regenerated ATP by using exogenous polyphosphate. This recombinant could be used as a platform to produce valuable compounds in combination with thermostable phosphorylating or energy-requiring enzymes. In this work, we demonstrated the production of fructose 1,6-diphosphate from fructose and polyphosphate.

* Corresponding author. Mailing address: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8530, Japan. Phone: 81-82-424-7758. Fax: 81-82-424-7047. E-mail: akuroda{at}hiroshima-u.ac.jp

{triangledown} Published ahead of print on 6 July 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, September 2007, p. 5676-5678, Vol. 73, No. 17
0099-2240/07/$08.00+0     doi:10.1128/AEM.00278-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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