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Applied and Environmental Microbiology, September 2007, p. 5949-5950, Vol. 73, No. 18
0099-2240/07/$08.00+0     doi:10.1128/AEM.00668-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Sensitivity of Nested PCR in the Detection of Low Numbers of Giardia lamblia Cysts{triangledown}

Kathryn M. Miller and Charles R. Sterling*

Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721

Received 23 March 2007/ Accepted 13 July 2007

Nested PCR was performed on individually isolated Giardia lamblia cysts in replicates of 50 for sets of 1, 2, 3, 4, 5, 7, and 10 cysts. Amplification ranged from 80% for 1 cyst to 100% for 10 cysts. The results suggest that nested PCR is well adapted for G. lamblia single-cyst detection.

* Corresponding author. Mailing address: University of Arizona, Department of Veterinary Science and Microbiology, Bldg. 90, 1117 E. Lowell St., Tucson, AZ 85721. Phone: (520) 621-4580. Fax: (520) 621-3588. E-mail: csterlin{at}u.arizona.edu

{triangledown} Published ahead of print on 20 July 2007.

Applied and Environmental Microbiology, September 2007, p. 5949-5950, Vol. 73, No. 18
0099-2240/07/$08.00+0     doi:10.1128/AEM.00668-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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