AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
AEM.02537-06v1
73/18/5954    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Manor, Y.
Right arrow Articles by Shulman, L. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Manor, Y.
Right arrow Articles by Shulman, L. M.
Agricola
Right arrow Articles by Manor, Y.
Right arrow Articles by Shulman, L. M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, September 2007, p. 5954-5958, Vol. 73, No. 18
0099-2240/07/$08.00+0     doi:10.1128/AEM.02537-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Advanced Environmental Surveillance and Molecular Analyses Indicate Separate Importations Rather than Endemic Circulation of Wild Type 1 Poliovirus in Gaza District in 2002{triangledown}

Y. Manor,1 S. Blomqvist,2 D. Sofer,1 J. Alfandari,1 T. Halmut,1 B. Abramovitz,1 E. Mendelson,1,3 and L. M. Shulman1*

Central Virology Laboratory, Public Health Services, Israel Ministry of Health, Chaim Sheba Medical Center, Tel Hashomer, Israel,1 Enterovirus Laboratory, Viral Diseases and Immunology, National Public Health Institute (KTL), Helsinki, Finland,2 Faculty of Life Sciences, Bar Ilan University, Ramat Gan, Israel3

Received 31 October 2006/ Accepted 19 July 2007

An improved sewage surveillance algorithm (sample acquisition, processing, and molecular analysis) for wild and vaccine-derived polioviruses was developed and validated. It was based on plaque isolation with sensitive and high-throughput methods. The molecular analysis included sequencing; a comparison of the type, rate, and distribution of nucleotide substitutions with a profile for outbreaks evolving from a single progenitor; and phylogenetic analysis for relative similarity. The analyses revealed that two environmental wild type 1 isolates from the Gaza district in 2002 were imported separately, most likely from Egyptian southern governorates, and were not linked by endemic circulation. These findings illustrate the continuous spreading potential of wild-type poliovirus and underscore the value of extensive environmental surveillance employing advanced molecular analysis to monitor wild poliovirus in poliomyelitis-free regions.

* Corresponding author. Mailing address: Laboratory of Environmental Virology, Public Health Services, Israel Ministry of Health, Central Virology Laboratory, Chaim Sheba Medical Center, Tel Hashomer, Israel 52621. Phone: 972-3-5302341. Fax: 972-3-5302457. E-mail: cvlsheba{at}netvision.net.il

{triangledown} Published ahead of print on 27 July 2007.

Applied and Environmental Microbiology, September 2007, p. 5954-5958, Vol. 73, No. 18
0099-2240/07/$08.00+0     doi:10.1128/AEM.02537-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2007 by the American Society for Microbiology. All rights reserved.