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Applied and Environmental Microbiology, September 2007, p. 5962-5967, Vol. 73, No. 18
0099-2240/07/$08.00+0     doi:10.1128/AEM.00817-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Comparison of Different Denaturing Gradient Gel Electrophoresis Primer Sets for the Study of Marine Bacterioplankton Communities{triangledown} ,{dagger}

Olga Sánchez,1* Josep M. Gasol,2 Ramon Massana,2 Jordi Mas,1 and Carlos Pedrós-Alió2

Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain,1 Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar CMIMA-CSIC, 08003 Barcelona, Spain2

Received 11 April 2007/ Accepted 15 July 2007

An annual seasonal cycle of composition of a bacterioplankton community in an oligotrophic coastal system was studied by denaturing gradient gel electrophoresis (DGGE) using five different primer sets. Analysis of DGGE fingerprints showed that primer set 357fGC-907rM grouped samples according to seasons. Additionally, we used the set of 16S rRNA genes archived in the RDPII database to check the percentage of perfect matches of each primer for the most abundant bacterial groups inhabiting coastal plankton communities. Overall, primer set 357fGC-907rM was the most suitable for the routine use of PCR-DGGE analyses in this environment.

* Corresponding author. Mailing address: Departament de Genètica i Microbiologia, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain. Phone: 34-935813011. Fax: 34-935812387. E-mail: olga.sanchez{at}uab.es

{triangledown} Published ahead of print on 27 July 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, September 2007, p. 5962-5967, Vol. 73, No. 18
0099-2240/07/$08.00+0     doi:10.1128/AEM.00817-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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