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 Previous Article

Applied and Environmental Microbiology, September 2007, p. 5971-5974, Vol. 73, No. 18
0099-2240/07/$08.00+0     doi:10.1128/AEM.00643-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Relative Abundances of Proteobacterial Membrane-Bound and Periplasmic Nitrate Reductases in Selected Environments{triangledown} ,{dagger}

D. Bru, A. Sarr, and L. Philippot*

INRA, University of Burgundy, Soil and Environmental Microbiology, CMSE, 17 rue Sully, B.P. 86510, 21065 Dijon Cedex, France

Received 21 March 2007/ Accepted 3 July 2007

Dissimilatory nitrate reduction is catalyzed by a membrane-bound and a periplasmic nitrate reductase. We set up a real-time PCR assay to quantify these two enzymes, using the narG and napA genes, encoding the catalytic subunits of the two types of nitrate reductases, as molecular markers. The narG and napA gene copy numbers in DNA extracted from 18 different environments showed high variations, with most numbers ranging from 2 x 102 to 6.8 x 104 copies per ng of DNA. This study provides evidence that, in soil samples, the number of proteobacteria carrying the napA gene is often as high as that of proteobacteria carrying the narG gene. The high correlation observed between narG and napA gene copy numbers in soils suggests that the ecological roles of the corresponding enzymes might be linked.

* Corresponding author. Mailing address: INRA, University of Burgundy, Soil and Environmental Microbiology, CMSE, 17 rue Sully, B.P. 86510, 21065 Dijon Cedex, France. Phone: 33 3 80 69 33 46. Fax: 33 3 80 69 32 24. E-mail: philippo{at}dijon.inra.fr

{triangledown} Published ahead of print on 13 July 2007.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, September 2007, p. 5971-5974, Vol. 73, No. 18
0099-2240/07/$08.00+0     doi:10.1128/AEM.00643-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cheneby, D., Brauman, A., Rabary, B., Philippot, L. (2009). Differential Responses of Nitrate Reducer Community Size, Structure, and Activity to Tillage Systems. Appl. Environ. Microbiol. 75: 3180-3186 [Abstract] [Full Text]  


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