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Applied and Environmental Microbiology, October 2007, p. 6296-6298, Vol. 73, No. 19
0099-2240/07/$08.00+0     doi:10.1128/AEM.00325-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Use of a Disposable Water Filter for Prevention of False-Positive Results due to Nontuberculosis Mycobacteria in a Clinical Laboratory Performing Routine Acid-Fast Staining for Tuberculosis{triangledown}

Hui-Zin Tu,1,2 Chiao-Shan Chen,1,2 Tsi-Shu Huang,1 Wen-Kuei Huang,1 Yao-shen Chen,1,2 Yung-ching Liu,1 and Yusen Eason Lin2*

Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan,1 National Kaohsiung Normal University, Kaohsiung, Taiwan2

Received 9 February 2007/ Accepted 29 July 2007

A point-of-use 0.2-µm filter was evaluated for elimination of nontuberculosis mycobacteria in laboratory water to reduce false-positive acid-fast bacillus staining results. Use of the point-of-use filter can significantly reduce the false-positive rate to 1.2% compared to samples treated with tap water (10.7%) and deionized water (8.7%).

* Corresponding author. Mailing address: Graduate Institute of Environmental Education, National Kaohsiung Normal University, 62 Shen-chong Rd., Yanchao, Kaohsiung, Taiwan 824. Phone: 886-7-6051036. Fax: 886-7-6051379. E-mail: easonlin{at}nknucc.nknu.edu.tw

{triangledown} Published ahead of print on 3 August 2007.

Applied and Environmental Microbiology, October 2007, p. 6296-6298, Vol. 73, No. 19
0099-2240/07/$08.00+0     doi:10.1128/AEM.00325-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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