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Applied and Environmental Microbiology, October 2007, p. 6299-6304, Vol. 73, No. 19
0099-2240/07/$08.00+0     doi:10.1128/AEM.00961-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Multiplex PCR for Simultaneous Detection of Bacteria of the Genus Listeria, Listeria monocytogenes, and Major Serotypes and Epidemic Clones of L. monocytogenes{triangledown}

Yi Chen* and Stephen J. Knabel

Food Science Department, The Pennsylvania State University, University Park, Pennsylvania 16802

Received 30 April 2007/ Accepted 2 August 2007

A multiplex PCR assay which combines detection of bacteria of the genus Listeria, Listeria monocytogenes serotypes 1/2a and 4b, and epidemic clones I, II, and III of L. monocytogenes was developed. The assay provides a rapid, reliable, and inexpensive method for screening and subgrouping this important food-borne pathogen.

* Corresponding author. Mailing address: The Pennsylvania State University, Food Science Department, 436 Food Science Building, University Park, PA 16802. Phone: (814) 865-7106. Fax: (814) 863-6132. E-mail: yxc211{at}psu.edu

{triangledown} Published ahead of print on 10 August 2007.

Applied and Environmental Microbiology, October 2007, p. 6299-6304, Vol. 73, No. 19
0099-2240/07/$08.00+0     doi:10.1128/AEM.00961-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kim, J.-W., Kathariou, S. (2009). Temperature-Dependent Phage Resistance of Listeria monocytogenes Epidemic Clone II. Appl. Environ. Microbiol. 75: 2433-2438 [Abstract] [Full Text]  


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