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Applied and Environmental Microbiology, October 2007, p. 6305-6312, Vol. 73, No. 19
0099-2240/07/$08.00+0     doi:10.1128/AEM.00715-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Identification of Genes That Confer Sediment Fitness to Desulfovibrio desulfuricans G20{triangledown}

Qingwei Luo,1 Jennifer L. Groh,1 Jimmy D. Ballard,2 and Lee R. Krumholz1*

Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019,1 Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 731902

Received 29 March 2007/ Accepted 6 August 2007

Signature-tagged mutants of Desulfovibrio desulfuricans G20 were screened, and 97 genes crucial for sediment fitness were identified. These genes belong to functional categories including signal transduction, binding and transport, insertion elements, and others. Mutants with mutations in genes encoding proteins involved in amino acid biosynthesis, hydrogenase activity, and DNA repair were further characterized.

* Corresponding author. Mailing address: University of Oklahoma, Department of Botany and Microbiology, 770 Van Vleet Oval, Norman, OK 73019. Phone: (405) 325-0437. Fax: (405) 325-7619. E-mail: krumholz{at}ou.edu

{triangledown} Published ahead of print on 17 August 2007.

Applied and Environmental Microbiology, October 2007, p. 6305-6312, Vol. 73, No. 19
0099-2240/07/$08.00+0     doi:10.1128/AEM.00715-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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