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Applied and Environmental Microbiology, January 2007, p. 451-455, Vol. 73, No. 2
0099-2240/07/$08.00+0     doi:10.1128/AEM.02087-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Sulfur-Oxidizing Bacteria in Soap Lake (Washington State), a Meromictic, Haloalkaline Lake with an Unprecedented High Sulfide Content{triangledown}

Dimitry Y. Sorokin,1,2* Mirjam Foti,1 Holly C. Pinkart,3 and Gerard Muyzer1

Department of Biotechnology, Delft University of Technology, 2628 BC Delft, The Netherlands,1 Winogradsky Institute of Microbiology, Russian Academy of Sciences, 117811 Moscow, Russia,2 Department of Biological Sciences, Central Washington University, Ellensburg, Washington 989263

Received 4 September 2006/ Accepted 7 November 2006

Culture-dependent and -independent techniques were used to study the diversity of chemolithoautotrophic sulfur-oxidizing bacteria in Soap Lake (Washington State), a meromictic, haloalkaline lake containing an unprecedentedly high sulfide concentration in the anoxic monimolimnion. Both approaches revealed the dominance of bacteria belonging to the genus Thioalkalimicrobium, which are common inhabitants of soda lakes. A dense population of Thioalkalimicrobium (up to 107 cells/ml) was found at the chemocline, which is characterized by a steep oxygen-sulfide gradient. Twelve Thioalkalimicrobium strains exhibiting three different phenotypes were isolated in pure culture from various locations in Soap Lake. The isolates fell into two groups according to 16S rRNA gene sequence analysis. One of the groups was closely related to T. cyclicum, which was isolated from Mono Lake (California), a transiently meromictic, haloalkaline lake. The second group, consisting of four isolates, was phylogenetically and phenotypically distinct from known Thioalkalimicrobium species and unique to Soap Lake. It represented a new species, for which we suggest the name Thioalkalimicrobium microaerophilum sp. nov.

* Corresponding author. Mailing address: Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/2, 117811 Moscow, Russia. Phone: (7095)1350109. Fax: (7095)1356530. E-mail: soroc{at}inmi.host.ru.

{triangledown} Published ahead of print on 17 November 2006.

Applied and Environmental Microbiology, January 2007, p. 451-455, Vol. 73, No. 2
0099-2240/07/$08.00+0     doi:10.1128/AEM.02087-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Dimitriu, P. A., Pinkart, H. C., Peyton, B. M., Mormile, M. R. (2008). Spatial and Temporal Patterns in the Microbial Diversity of a Meromictic Soda Lake in Washington State. Appl. Environ. Microbiol. 74: 4877-4888 [Abstract] [Full Text]  
  • (2007). List of new names and new combinations previously effectively, but not validly, published. Int. J. Syst. Evol. Microbiol. 57: 893-897 [Abstract] [Full Text]  


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