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Applied and Environmental Microbiology, January 2007, p. 659-662, Vol. 73, No. 2
0099-2240/07/$08.00+0     doi:10.1128/AEM.02130-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Comparison of Primer Sets for Use in Automated Ribosomal Intergenic Spacer Analysis of Aquatic Bacterial Communities: an Ecological Perspective{triangledown}

Stuart E. Jones,1 Ashley L. Shade,2 Katherine D. McMahon,1,2,3 and Angela D. Kent3,4*

Limnology and Marine Science Program,1 Microbiology Doctoral Training Program,2 Department of Civil and Environmental Engineering,3 Center for Limnology, University of Wisconsin—Madison, Madison, Wisconsin 537064

Received 8 September 2006/ Accepted 13 November 2006

Two primer sets for automated ribosomal intergenic spacer analysis (ARISA) were used to assess the bacterial community composition (BCC) in Lake Mendota, Wisconsin, over 3 years. Correspondence analysis revealed differences in community profiles generated by different primer sets, but overall ecological patterns were conserved in each case. ARISA is a powerful tool for evaluating BCC change through space and time, regardless of the specific primer set used.

* Corresponding author. Present address: Department of Natural Resources and Environmental Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801. Phone: (217) 333-4216. Fax: (217) 244-3219. E-mail: akent{at}uiuc.edu.

{triangledown} Published ahead of print on 22 November 2006.

Applied and Environmental Microbiology, January 2007, p. 659-662, Vol. 73, No. 2
0099-2240/07/$08.00+0     doi:10.1128/AEM.02130-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kara, E., Shade, A. (2009). Temporal Dynamics of South End Tidal Creek (Sapelo Island, Georgia) Bacterial Communities. Appl. Environ. Microbiol. 75: 1058-1064 [Abstract] [Full Text]  


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