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Applied and Environmental Microbiology, October 2007, p. 6678-6681, Vol. 73, No. 20
0099-2240/07/$08.00+0     doi:10.1128/AEM.00986-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Fine-Scale Genetic Diversity among Burkholderia pseudomallei Soil Isolates in Northeast Thailand{triangledown}

Jana M. U'Ren,1 Heidie Hornstra,1 Talima Pearson,1 James M. Schupp,1 Benjamin Leadem,1 Shalamar Georgia,1 Rasana W. Sermswan,2 and Paul Keim1*

Northern Arizona University, Center for Microbial Genetics and Genomics, Flagstaff, Arizona 86011,1 Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand2

Received 2 May 2007/ Accepted 15 August 2007

Burkholderia pseudomallei soil isolates from northeast Thailand were genotyped using multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) and multilocus sequence typing (MLST). MLVA identified 19 genotypes within three clades, while MLST revealed two genotypes. These close genetic relationships imply a recent colonization followed by localized expansion, similar to what occurs in an outbreak situation.

* Corresponding author. Mailing address: Northern Arizona University, Department of Biological Sciences, Box 5640, Flagstaff, AZ 86011-5640. Phone: (928) 523-1078. Fax: (928) 523-0639. E-mail: Paul.Keim{at}nau.edu

{triangledown} Published ahead of print on 24 August 2007.

Applied and Environmental Microbiology, October 2007, p. 6678-6681, Vol. 73, No. 20
0099-2240/07/$08.00+0     doi:10.1128/AEM.00986-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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