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Applied and Environmental Microbiology, November 2007, p. 6697-6704, Vol. 73, No. 21
0099-2240/07/$08.00+0     doi:10.1128/AEM.00535-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Retrospective Characterization of a Vaccine-Derived Poliovirus Type 1 Isolate from Sewage in Greece{triangledown}

Evaggelos Dedepsidis,1 Zaharoula Kyriakopoulou,1 Vaia Pliaka,1 Christine Kottaridi,1 Eugenia Bolanaki,1 Stamatina Levidiotou-Stefanou,2 Dimitri Komiotis,1 and Panayotis Markoulatos1*

Department of Biochemistry & Biotechnology, Microbiology-Virology Laboratory, University of Thessaly, 26 Ploutonos & Aiolou Str., Larisa 41221, Greece,1 Department of Microbiology, Medical School, University of Ioannina, Ioannina, Greece2

Received 8 March 2007/ Accepted 17 July 2007

Retrospective molecular and phenotypic characterization of a vaccine-derived poliovirus (VDPV) type 1 isolate (7/b/97) isolated from sewage in Athens, Greece, in 1997 is reported. VP1 sequencing of this isolate revealed 1.87% divergence from the VP1 region of reference strain Sabin 1, while further genomic characterization of isolate 7/b/97 revealed a recombination event in the nonstructural part of the genome between a vaccine strain and a nonvaccine strain probably belonging to Enterovirus species C. Amino acid substitutions commonly found in previous studies were identified in the capsid coding region of the isolate, while most of the attenuation and temperature sensitivity determinants were reverted. The ultimate source of isolate 7/b/97 is unknown. The recovery of such a highly divergent derivative of a vaccine strain emphasizes the need for urgent implementation of environmental surveillance as a supportive procedure in the polio surveillance system even in countries with high rates of OPV coverage in order to prevent cases or even outbreaks of poliomyelitis that otherwise would be inevitable.

* Corresponding author. Mailing address: Department of Biochemistry & Biotechnology, University of Thessaly, 26 Ploutonos & Aiolou Str., Larissa 41221, Greece. Phone: 302410565274. Fax: 302410565294. E-mail: Markoulatos{at}bio.uth.gr

{triangledown} Published ahead of print on 7 September 2007.

Applied and Environmental Microbiology, November 2007, p. 6697-6704, Vol. 73, No. 21
0099-2240/07/$08.00+0     doi:10.1128/AEM.00535-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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